Steven Yukl, MD

Telwatte S, Kim P, Chen TH, Milush JM, Somsouk M, Deeks SG, Hunt PW, Wong JK, Yukl SA. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents. 2020. PMID: 32910065

Timmons A, Fray E, Kumar M, Wu F, Dai W, Bullen CK, Kim P, Hetzel C, Yang C, Beg S, Lai J, Pomerantz JL, Yukl SA, Siliciano JD, Siliciano RF. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo. 2020. PMID: 32571938

Hsiao F, Frouard J, Gramatica A, Xie G, Telwatte S, Lee GQ, Roychoudhury P, Schwarzer R, Luo X, Yukl SA, Lee S, Hoh R, Deeks SG, Jones RB, Cavrois M, Greene WC, Roan NR. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection. 2020. PMID: 32353080

Lee SA, Telwatte S, Hatano H, Kashuba ADM, Cottrell ML, Hoh R, Liegler TJ, Stephenson S, Somsouk M, Hunt PW, Deeks SG, Yukl S, Savic RM. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay. 2020. PMID: 32168200

Telwatte S, Morón-López S, Aran D, Kim P, Hsieh C, Joshi S, Montano M, Greene WC, Butte AJ, Wong JK, Yukl SA. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency. 2019. PMID: 31711503

Jiang G, Maverakis E, Cheng MY, Elsheikh MM, Deleage C, Méndez-Lagares G, Shimoda M, Yukl SA, Hartigan-O'Connor DJ, Thompson GR, Estes JD, Wong JK, Dandekar S. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate. 2019. PMID: 30944245

Moron-Lopez S, Kim P, SøGaard OS, Tolstrup M, Wong JK, Yukl SA. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals. 2018. PMID: 30531314

Telwatte S, Lee S, Somsouk M, Hatano H, Baker C, Kaiser P, Kim P, Chen TH, Milush J, Hunt PW, Deeks SG, Wong JK, Yukl SA. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency. 2018. PMID: 30440043

Yukl SA, Kaiser P, Kim P, Telwatte S, Joshi SK, Vu M, Lampiris H, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing. 2018. PMID: 29491188

Jiang G, Nguyen D, Archin NM, Yukl SA, Méndez-Lagares G, Tang Y, Elsheikh MM, Thompson GR, Hartigan-O'Connor DJ, Margolis DM, Wong JK, Dandekar S. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. HIV latency is reversed by ACSS2-driven histone crotonylation. 2018. PMID: 29457784

. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Anatomic Compartments as a Barrier to HIV Cure. 2018. PMID:

Henrich TJ, Hatano H, Bacon O, Hogan LE, Rutishauser R, Hill A, Kearney MF, Anderson EM, Buchbinder SP, Cohen SE, Abdel-Mohsen M, Pohlmeyer CW, Fromentin R, Hoh R, Liu AY, McCune JM, Spindler J, Metcalf-Pate K, Hobbs KS, Thanh C, Gibson EA, Kuritzkes DR, Siliciano RF, Price RW, Richman DD, Chomont N, Siliciano JD, Mellors JW, Yukl SA, Blankson JN, Liegler T, Deeks SG. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study. Volume 14 of Issue 11. 2017. PMID: 29112956

Trapecar M, Khan S, Roan NR, Chen TH, Telwatte S, Deswal M, Pao M, Somsouk M, Deeks SG, Hunt PW, Yukl S, Sanjabi S. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. Volume 33 of Issue S1. 2017. PMID: 28882052

Khan S, Telwatte S, Trapecar M, Yukl S, Sanjabi S. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure. Volume 33 of Issue S1. 2017. PMID: 28882067

Cockerham LR, Yukl SA, Harvill K, Somsouk M, Joshi SK, Sinclair E, Liegler T, Hoh R, Lyons S, Hunt PW, Rupert A, Sereti I, Morcock DR, Rhodes A, Emson C, Hellerstein MK, Estes JD, Lewin S, Deeks SG, Hatano H. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals. Volume 2 of Issue 3. 2017. PMID: 28936485

Koelsch KK, Rasmussen TA, Hey-Nguyen WJ, Pearson C, Xu Y, Bailey M, Marks KH, Sasson SC, Taylor MS, Tantau R, Obeid S, Milner B, Morrissey O, Pinto AN, Suzuki K, Busch MP, Keating SM, Kaiser P, Yukl S, Wong JK, Hiener BM, Palmer S, Zaunders J, Post JJ, Chan DJ, Avery S, Milliken ST, Kelleher AD, Lewin SR, Cooper DA. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals. Volume 75 of Issue 3. 2017. PMID: 28350553

. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Exploring HIV latency using transcription profiling. 2017. PMID:

Kaiser P, Joshi SK, Kim P, Li P, Liu H, Rice AP, Wong JK, Yukl SA. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Assays for precise quantification of total (including short) and elongated HIV-1 transcripts. Volume 242. 2016. PMID: 28034670

Lee SA, Bacchetti P, Chomont N, Fromentin R, Lewin SR, O'Doherty U, Palmer S, Richman DD, Siliciano JD, Yukl SA, Deeks SG, Burbelo PD. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy. Volume 11 of Issue 8. 2016. PMID: 27483366

Wong JK, Yukl SA. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Tissue reservoirs of HIV. Volume 11 of Issue 4. 2016. PMID: 27259045

Li P, Kaiser P, Lampiris HW, Kim P, Yukl SA, Havlir DV, Greene WC, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation. Volume 22 of Issue 7. 2016. PMID: 27294875

Yukl SA, Shergill AK, Girling V, Li Q, Killian M, Epling L, Li P, Kaiser P, Haase A, Havlir DV, McQuaid K, Sinclair E, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults. Volume 10 of Issue 3. 2015. PMID: 25811360

. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Anatomic Compartments as a Barrier to HIV Cure. 2015. PMID:

Cockerham LR, Siliciano JD, Sinclair E, O'Doherty U, Palmer S, Yukl SA, Strain MC, Chomont N, Hecht FM, Siliciano RF, Richman DD, Deeks SG. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells. Volume 9 of Issue 10. 2014. PMID: 25340755

Li P, Fujimoto K, Bourguingnon L, Yukl S, Deeks S, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells. Volume 92 of Issue 9. 2014. PMID: 24957217

Wang C, Abdel-Mohsen M, Strain MC, Lada SM, Yukl S, Cockerham LR, Pilcher CD, Hecht FM, Sinclair E, Liegler T, Richman DD, Deeks SG, Pillai SK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy. Volume 210 of Issue 11. 2014. PMID: 24935955

Yukl SA, Kaiser P, Kim P, Li P, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR. Volume 56 of Issue 4. 2014. PMID: 24724845

Dunham RM, Vujkovic-Cvijin I, Yukl SA, Broadhurst MJ, Loke P, Albright RG, Wong JK, Lederman MM, Somsouk M, Hunt PW, Martin JN, Deeks SG, McCune JM. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Discordance between peripheral and colonic markers of inflammation during suppressive ART. Volume 65 of Issue 2. 2014. PMID: 24121758

Yukl SA, Sinclair E, Somsouk M, Hunt PW, Epling L, Killian M, Girling V, Li P, Havlir DV, Deeks SG, Wong JK, Hatano H. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells. Volume 28 of Issue 3. 2014. PMID: 24322272

Hatano H, Yukl SA, Ferre AL, Graf EH, Somsouk M, Sinclair E, Abdel-Mohsen M, Liegler T, Harvill K, Hoh R, Palmer S, Bacchetti P, Hunt PW, Martin JN, McCune JM, Tracy RP, Busch MP, O'Doherty U, Shacklett BL, Wong JK, Deeks SG. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers. Volume 9 of Issue 10. 2013. PMID: 24130489

Hatano H, Somsouk M, Sinclair E, Harvill K, Gilman L, Cohen M, Hoh R, Hunt PW, Martin JN, Wong JK, Deeks SG, Yukl SA. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers. Volume 27 of Issue 14. 2013. PMID: 24157906

Yukl SA, Shergill AK, Ho T, Killian M, Girling V, Epling L, Li P, Wong LK, Crouch P, Deeks SG, Havlir DV, McQuaid K, Sinclair E, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence. Volume 208 of Issue 8. 2013. PMID: 23852128

Yukl SA, Boritz E, Busch M, Bentsen C, Chun TW, Douek D, Eisele E, Haase A, Ho YC, Hütter G, Justement JS, Keating S, Lee TH, Li P, Murray D, Palmer S, Pilcher C, Pillai S, Price RW, Rothenberger M, Schacker T, Siliciano J, Siliciano R, Sinclair E, Strain M, Wong J, Richman D, Deeks SG. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient. Volume 9 of Issue 5. 2013. PMID: 23671416

Eriksson S, Graf EH, Dahl V, Strain MC, Yukl SA, Lysenko ES, Bosch RJ, Lai J, Chioma S, Emad F, Abdel-Mohsen M, Hoh R, Hecht F, Hunt P, Somsouk M, Wong J, Johnston R, Siliciano RF, Richman DD, O'Doherty U, Palmer S, Deeks SG, Siliciano JD. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies. Volume 9 of Issue 2. 2013. PMID: 23459007

Pillai SK, Abdel-Mohsen M, Guatelli J, Skasko M, Monto A, Fujimoto K, Yukl S, Greene WC, Kovari H, Rauch A, Fellay J, Battegay M, Hirschel B, Witteck A, Bernasconi E, Ledergerber B, Günthard HF, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo. Volume 109 of Issue 8. 2012. PMID: 22315404

Yukl SA, Li P, Fujimoto K, Lampiris H, Lu CM, Hare CB, Deeks SG, Liegler T, Pandori M, Havlir DV, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter. Volume 175 of Issue 2. 2011. PMID: 21536073

. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut. 2010. PMID:

Yukl SA, Shergill AK, McQuaid K, Gianella S, Lampiris H, Hare CB, Pandori M, Sinclair E, Günthard HF, Fischer M, Wong JK, Havlir DV. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy. Volume 24 of Issue 16. 2010. PMID: 20827162

Yukl SA, Gianella S, Sinclair E, Epling L, Li Q, Duan L, Choi AL, Girling V, Ho T, Li P, Fujimoto K, Lampiris H, Hare CB, Pandori M, Haase AT, Günthard HF, Fischer M, Shergill AK, McQuaid K, Havlir DV, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy. Volume 202 of Issue 10. 2010. PMID: 20939732

Li P, Ruel T, Fujimoto K, Hatano H, Yukl S, Eller LA, Liegler T, Kamya M, Gassasira A, Dorsey G, Rosenthal PJ, Havlir DV, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes. Volume 170 of Issue 1-2. 2010. PMID: 20863855

Yukl S, Pillai S, Li P, Chang K, Pasutti W, Ahlgren C, Havlir D, Strain M, Günthard H, Richman D, Rice AP, Daar E, Little S, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity. Volume 387 of Issue 1. 2009. PMID: 19268337

Yukl S, Wong JK. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. Blood and guts and HIV: preferential HIV persistence in GI mucosa. Volume 197 of Issue 5. 2008. PMID: 18260765

Berrozpe G, Timokhina I, Yukl S, Tajima Y, Ono M, Zelenetz AD, Besmer P. Mechanistic differences underlying HIV latency in the gut and blood contribute to differential responses to latency-reversing agents.., HSF1 inhibition attenuates HIV-1 latency reversal mediated by several candidate LRAs In Vitro and Ex Vivo.., Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection.., Antiretroviral Therapy Concentrations Differ in Gut vs. Lymph Node Tissues and Are Associated With HIV Viral Transcription by a Novel RT-ddPCR Assay.., Heterogeneity in HIV and cellular transcription profiles in cell line models of latent and productive infection: implications for HIV latency.., Disruption of latent HIV in vivo during the clearance of actinic keratosis by ingenol mebutate.., Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals.., Gut and blood differ in constitutive blocks to HIV transcription, suggesting tissue-specific differences in the mechanisms that govern HIV latency.., HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing.., HIV latency is reversed by ACSS2-driven histone crotonylation.., Anatomic Compartments as a Barrier to HIV Cure.., HIV-1 persistence following extremely early initiation of antiretroviral therapy (ART) during acute HIV-1 infection: An observational study.., An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.., Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure.., A Randomized Controlled Trial of Lisinopril to Decrease Lymphoid Fibrosis in Antiretroviral-Treated, HIV-infected Individuals.., Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals.., Exploring HIV latency using transcription profiling.., Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.., Anti-HIV Antibody Responses and the HIV Reservoir Size during Antiretroviral Therapy.., Tissue reservoirs of HIV.., Stimulating the RIG-I pathway to kill cells in the latent HIV reservoir following viral reactivation.., Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults.., Anatomic Compartments as a Barrier to HIV Cure.., CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells.., Exogenous and endogenous hyaluronic acid reduces HIV infection of CD4(+) T cells.., Decreased HIV type 1 transcription in CCR5-?32 heterozygotes during suppressive antiretroviral therapy.., Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR.., Discordance between peripheral and colonic markers of inflammation during suppressive ART.., A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.., Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.., Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers.., The distribution of HIV DNA and RNA in cell subsets differs in gut and blood of HIV-positive patients on ART: implications for viral persistence.., Challenges in detecting HIV persistence during potentially curative interventions: a study of the Berlin patient.., Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies.., Role of retroviral restriction factors in the interferon-a-mediated suppression of HIV-1 in vivo.., Modification of the Abbott RealTime assay for detection of HIV-1 plasma RNA viral loads less than one copy per milliliter.., CD4+ T cell reconstitution, T cell activation, and memory T cell subset composition in blood and gut of HIV-negative and ART-suppressed HIV-positive patients: implications for HIV persistence in the gut.., Effect of raltegravir-containing intensification on HIV burden and T-cell activation in multiple gut sites of HIV-positive adults on suppressive antiretroviral therapy.., Differences in HIV burden and immune activation within the gut of HIV-positive patients receiving suppressive antiretroviral therapy.., Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes.., Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.., Blood and guts and HIV: preferential HIV persistence in GI mucosa.., The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.. The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit. 1999. PMID: 10515869