David Pearce, MD

Professor

EDUCATION
College: University of California, Berkeley BA (Mathematics)
Medical School: University of Chicago, Pritzker School of Medicine, MD
Internal Medicine Residency: Harbor-UCLA Medical Center
Nephrology Fellowship: University of California, San Francisco

Dr. Pearce's research focuses on understanding the cellular and molecular mechanisms underlying hormone-regulated ion transport in the kidney tubules. His lab has cloned and characterized several key regulatory proteins involved in the control of epithelial Na+ and K+ transport. Notably, SGK1 is a serine-threonine signaling kinase, which mediates the effects of multiple hormones, and is necessary for normal Na+ and K+ homeostasis. Defects in SGK1 result in pseudohypoaldosteronism (Na+ wasting and inability to excrete K+ despite high aldosterone levels).

His group has made seminal contributions to our understanding of how the effects of SGK1 and other signaling proteins are integrated to achieve specific regulation of ion transport. Recently, his lab identified a large multiprotein complex, which integrates manifold inputs that impact on Na+ transport in kidney tubule cells. The principal goal of this basic research is to identify points of entry for treatment of diseases such as hypertension and metabolic syndrome. Already, SGK1 inhibitors are in preclinical testing for the treatment of salt sensitive hypertension.

Dr. Pearce's research is funded by two R01's from the NIDDK, and several Foundation awards.

Dr. Pearce is Professor of Medicine and Cellular & Molecular Pharmacology at UCSF, and Chief of Nephrology at San Francisco General Hospital, one of the core teaching hospitals affiliated with UCSF.

Education
Post-Doc Fellow/Scholar, 1989 - Medicine, University of California, San Francisco
Residency, - , University of California, San Francisco
Publications

  1. Verouti S, Aeschlimann G, Wang Q, Del Olmo DA, Peyter AC, Menétrey S, Winter DV, Odermatt A, Pearce D, Hummler E, Vanderriele PE. Salt-sensitive hypertension in GR mutant rats is associated with altered plasma polyunsaturated fatty acid levels and aortic vascular reactivity. Pflugers Archiv : European journal of physiology 2024. PMID: 39256246


  2. Demko J, Saha B, Takagi E, Mannis A, Weber R, Pearce D. Coordinated Regulation of Renal Glucose Reabsorption and Gluconeogenesis by mTORC2 and Potassium. bioRxiv : the preprint server for biology 2024. PMID: 38979219


  3. Demko J, Weber R, Pearce D, Saha B. Aldosterone-independent regulation of K+ secretion in the distal nephron. Current opinion in nephrology and hypertension 2024. PMID: 38888034


  4. John Demko, Bidisha Saha, Enzo Takagi, Anna Manis, Phillip Richman, David Pearce. Renal tubule mTORC2 deletion increases gluconeogenesis and urinary glucose excretion. Physiology 2023. PMID:


  5. Bidisha Saha, Enzo Takagi, John Demko, Anna Manis, David Pearce. Site-specific activity of mTORC2 in regulating renal K+ secretion to maintain ion homeostasis. Physiology 2023. PMID:


  6. Saha B, Shabbir W, Takagi E, Duan XP, Almeida Leite Dellova DC, Demko J, Manis A, Loffing-Cueni D, Loffing J, S?rensen MV, Wang WH, Pearce D. Potassium Activates mTORC2-dependent SGK1 Phosphorylation to Stimulate ENaC: Role in Rapid Renal Responses to Dietary Potassium. Journal of the American Society of Nephrology : JASN 2023. PMID: 36890646


  7. Saha B, Leite-Dellova DCA, Demko J, S?rensen MV, Takagi E, Gleason CE, Shabbir W, Pearce D. WNK1 is a chloride-stimulated scaffold that regulates mTORC2 activity and ion transport. Journal of cell science 2022. PMID: 36373794


  8. Parikh NI, Arowolo F, Durstenfeld MS, Nah G, Njoroge J, Vittinghoff E, Long CS, Ganz P, Pearce D, Hsue P, Wu AHS, Hajizadeh N, Liu KD, Lynch KL. Hospitalized Patients With COVID-19 Have Higher Plasma Aldosterone-Renin Ratio and Lower ACE Activity Than Controls. Journal of the Endocrine Society 2022. PMID: 36338506


  9. Yu Z, Chen J, Takagi E, Wang F, Saha B, Liu X, Joubert LM, Gleason CE, Jin M, Li C, Nowotny C, Agard D, Cheng Y, Pearce D. Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt. The Journal of biological chemistry 2022. PMID: 35926713


  10. Pearce D, Manis AD, Nesterov V, Korbmacher C. Regulation of distal tubule sodium transport: mechanisms and roles in homeostasis and pathophysiology. Pflugers Archiv : European journal of physiology 2022. PMID: 35895103


  11. Allu PKR, Paulo E, Bertholet AM, Situ G, Lee SH, Wu Y, Gleason CE, Saha B, Chawla A, Wang B, Pearce D. Role of mTORC2 in biphasic regulation of brown fat metabolism in response to mild and severe cold. The Journal of biological chemistry 2021. PMID: 33865855


  12. Stookey JD, Allu PKR, Chabas D, Pearce D, Lang F. Hypotheses about sub-optimal hydration in the weeks before coronavirus disease (COVID-19) as a risk factor for dying from COVID-19. Medical hypotheses 2020. PMID: 33254543


  13. Deise C. A. Leite Dellova, Bidisha Saha, Enzo Takagi, Waheed Shabbir, David Pearce. Regulation of ENaC by aldosterone and potassium. The FASEB Journal 2020. PMID:


  14. Waheed Shabbir, Catherine E. Gleason, Bidisha Saha, Deise C. A. Leite Dellova, David Pearce. mTOR Modulates Sodium and Potassium Conduction in Heterologous Expression System. The FASEB Journal 2020. PMID:


  15. Chen JL, Wang L, Yao XM, Zang YJ, Wang Y, Li ZJ, Pearce D, Wang H. Association of Urinary Plasminogen-Plasmin with Edema and Epithelial Sodium Channel Activation in Patients with Nephrotic Syndrome. American journal of nephrology 2019. PMID: 31269481


  16. Sørensen MV, Saha B, Jensen IS, Wu P, Ayasse N, Gleason CE, Svendsen SL, Wang WH, Pearce D. Potassium acts through mTOR to regulate its own secretion. JCI insight 2019. PMID: 31013253


  17. Gleason CE, Oses-Prieto JA, Li KH, Saha B, Situ G, Burlingame AL, Pearce D. Phosphorylation at distinct subcellular locations underlies specificity in mTORC2-mediated activation of SGK1 and Akt. Journal of cell science 2019. PMID: 30837283


  18. Bidisha Saha, Catherine Gleason, Peng Wu, Wen-Hui Wang, David Pearce. The mTORC2-SGK1 Signaling Module Mediates Local Sensing of Potassium in Kidney Tubule Principal Cells to Activate ENaC and Promote Potassium Secretion. The FASEB Journal 2019. PMID:


  19. Yuan YP, Zhao H, Peng LQ, Li ZF, Liu S, Yuan CY, Mwamunyi MJ, Pearce D, Yao LJ. The SGK3-triggered ubiquitin-proteasome degradation of podocalyxin (PC) and ezrin in podocytes was associated with the stability of the PC/ezrin complex. Cell death & disease 2018. PMID: 30385740


  20. Bidisha Saha, Peng Wu, Catherine E. Gleason, Wenhui Wang, David Pearce. Potassium Can Regulate Its Own Excretion in Collecting Duct Cells: Role of mTORC2, SGK1 and ENaC. The FASEB Journal 2018. PMID:


  21. Morze CV, Allu PKR, Chang GY, Marco-Rius I, Milshteyn E, Wang ZJ, Ohliger MA, Gleason CE, Kurhanewicz J, Vigneron DB, Pearce D. Non-invasive detection of divergent metabolic signals in insulin deficiency vs. insulin resistance in vivo. Scientific Reports 2018. PMID: 29391429


  22. Peng LQ, Zhao H, Liu S, Yuan YP, Yuan CY, Mwamunyi MJ, Pearce D, Yao LJ. Lack of serum- and glucocorticoid-inducible kinase 3 leads to podocyte dysfunction. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018. PMID: 28935820


  23. von Morze C, Ohliger MA, Marco-Rius I, Wilson DM, Flavell RR, Pearce D, Vigneron DB, Kurhanewicz J, Wang ZJ. Direct assessment of renal mitochondrial redox state using hyperpolarized 13 C-acetoacetate. Magnetic resonance in medicine 2018. PMID: 29314217


  24. Nelson T, Pearce DA, Kovács AD. Lack of specificity of antibodies raised against CLN3, the lysosomal/endosomal transmembrane protein mutated in juvenile Batten disease. Bioscience reports 2017. PMID: 29089465


  25. Rashmi P, Colussi G, Ng M, Wu X, Kidwai A, Pearce D. Glucocorticoid-induced leucine zipper protein regulates sodium and potassium balance in the distal nephron. Kidney international 2017. PMID: 28094030


  26. Marco-Rius I, von Morze C, Sriram R, Cao P, Chang GY, Milshteyn E, Bok RA, Ohliger MA, Pearce D, Kurhanewicz J, Larson PE, Vigneron DB, Merritt M. Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2-13 C]dihydroxyacetone. Magnetic resonance in medicine 2016. PMID: 27859575


  27. Meyer M, Kovács AD, Pearce DA. Decreased sensitivity of palmitoyl protein thioesterase 1-deficient neurons to chemical anoxia. Metabolic brain disease 2016. PMID: 27722792


  28. von Morze C, Chang GY, Larson PE, Shang H, Allu PK, Bok RA, Crane JC, Olson MP, Tan CT, Marco-Rius I, Nelson SJ, Kurhanewicz J, Pearce D, Vigneron DB. Detection of localized changes in the metabolism of hyperpolarized gluconeogenic precursors 13 C-lactate and 13 C-pyruvate in kidney and liver. Magnetic resonance in medicine 2016. PMID: 27098724


  29. Cornelius von Morze, Gene-Yuan Chang, Peder E Larson, Hong Shang, Prasanna KR Allu, Robert A Bok, Jason C Crane, Marram P Olson, Chou T Tan, Irene Marco-Rius, Sarah J Nelson, John Kurhanewicz, David Pearce, Daniel B Vigneron. Detecting Metabolism of Gluconeogenic Precursors Lactate and Pyruvate in Kidney & Liver with Hyperpolarized 13 C MRI. The FASEB Journal 2016. PMID:


  30. Marco-Rius I, Cao P, von Morze C, Merritt M, Moreno KX, Chang GY, Ohliger MA, Pearce D, Kurhanewicz J, Larson PE, Vigneron DB. Multiband spectral-spatial RF excitation for hyperpolarized [2-13 C]dihydroxyacetone 13 C-MR metabolism studies. Magnetic resonance in medicine 2016. PMID: 27017966


  31. Lang F, Pearce D. Regulation of the epithelial Na+ channel by the mTORC2/SGK1 pathway. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2015. PMID: 26163195


  32. Gleason CE, Frindt G, Cheng CJ, Ng M, Kidwai A, Rashmi P, Lang F, Baum M, Palmer LG, Pearce D. mTORC2 regulates renal tubule sodium uptake by promoting ENaC activity. The Journal of clinical investigation 2014. PMID: 25415435


  33. Miller JN, Kovács AD, Pearce DA. The novel Cln1(R151X) mouse model of infantile neuronal ceroid lipofuscinosis (INCL) for testing nonsense suppression therapy. Human molecular genetics 2014. PMID: 25205113


  34. Pearce D, Soundararajan R, Trimpert C, Kashlan OB, Deen PM, Kohan DE. Collecting duct principal cell transport processes and their regulation. Clinical journal of the American Society of Nephrology : CJASN 2014. PMID: 24875192


  35. Zhang X, Zhou Q, Chen L, Berger S, Wu H, Xiao Z, Pearce D, Zhou X, Zhang W. Mineralocorticoid receptor antagonizes Dot1a-Af9 complex to increase aENaC transcription. American journal of physiology. Renal physiology 2013. PMID: 24026182


  36. Getty A, Kovács AD, Lengyel-Nelson T, Cardillo A, Hof C, Chan CH, Pearce DA. Osmotic stress changes the expression and subcellular localization of the Batten disease protein CLN3. PloS one 2013. PMID: 23840424


  37. Miller JN, Chan CH, Pearce DA. The role of nonsense-mediated decay in neuronal ceroid lipofuscinosis. Human molecular genetics 2013. PMID: 23539563


  38. Kim HJ, Prasad V, Hyung SW, Lee ZH, Lee SW, Bhargava A, Pearce D, Lee Y, Kim HH. Plasma membrane calcium ATPase regulates bone mass by fine-tuning osteoclast differentiation and survival. The Journal of cell biology 2012. PMID: 23266958


  39. Schmid E, Bhandaru M, Nurbaeva MK, Yang W, Szteyn K, Russo A, Leibrock C, Tyan L, Pearce D, Shumilina E, Lang F. SGK3 regulates Ca(2+) entry and migration of dendritic cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2012. PMID: 23171960


  40. Soundararajan R, Ziera T, Koo E, Ling K, Wang J, Borden SA, Pearce D. Scaffold protein connector enhancer of kinase suppressor of Ras isoform 3 (CNK3) coordinates assembly of a multiprotein epithelial sodium channel (ENaC)-regulatory complex. The Journal of biological chemistry 2012. PMID: 22851176


  41. Kovács AD, Saje A, Wong A, Ramji S, Cooper JD, Pearce DA. Age-dependent therapeutic effect of memantine in a mouse model of juvenile Batten disease. Neuropharmacology 2012. PMID: 22683643


  42. Romero Y, Vuandaba M, Suarez P, Grey C, Calvel P, Conne B, Pearce D, de Massy B, Hummler E, Nef S. The Glucocorticoid-induced leucine zipper (GILZ) Is essential for spermatogonial survival and spermatogenesis. Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation 2012. PMID: 22571926


  43. Suarez PE, Rodriguez EG, Soundararajan R, Mérillat AM, Stehle JC, Rotman S, Roger T, Voirol MJ, Wang J, Gross O, Pétrilli V, Nadra K, Wilson A, Beermann F, Pralong FP, Maillard M, Pearce D, Chrast R, Rossier BC, Hummler E. The glucocorticoid-induced leucine zipper (gilz/Tsc22d3-2) gene locus plays a crucial role in male fertility. Molecular endocrinology (Baltimore, Md.) 2012. PMID: 22556341


  44. Soundararajan R, Lu M, Pearce D. Organization of the ENaC-regulatory machinery. Critical reviews in biochemistry and molecular biology 2012. PMID: 22506713


  45. David Pearce, Vivek Bhalla, John W. Funder, John B. Stokes. Chapter 6 Aldosterone Regulation of Ion Transport. 2012. PMID:


  46. Soundararajan R, Pearce D, Ziera T. The role of the ENaC-regulatory complex in aldosterone-mediated sodium transport. Molecular and cellular endocrinology 2011. PMID: 22101317


  47. Yao LJ, McCormick JA, Wang J, Yang KY, Kidwai A, Colussi GL, Boini KM, Birnbaum MJ, Lang F, German MS, Pearce D. Novel role for SGK3 in glucose homeostasis revealed in SGK3/Akt2 double-null mice. Molecular endocrinology (Baltimore, Md.) 2011. PMID: 21980074


  48. Finn R, Kovács AD, Pearce DA. Altered glutamate receptor function in the cerebellum of the Ppt1-/- mouse, a murine model of infantile neuronal ceroid lipofuscinosis. Journal of neuroscience research 2011. PMID: 21971706


  49. Lu M, Wang J, Ives HE, Pearce D. mSIN1 protein mediates SGK1 protein interaction with mTORC2 protein complex and is required for selective activation of the epithelial sodium channel. The Journal of biological chemistry 2011. PMID: 21757730


  50. Tim Ziera, Eric Koo, Rama Soundararajan, David Pearce. Identification of CNK3 in the Aldosterone-regulated ENaC Regulatory Complex. The FASEB Journal 2011. PMID:


  51. Bhandaru M, Kempe DS, Rotte A, Capuano P, Pathare G, Sopjani M, Alesutan I, Tyan L, Huang DY, Siraskar B, Judenhofer MS, Stange G, Pichler BJ, Biber J, Quintanilla-Martinez L, Wagner CA, Pearce D, Föller M, Lang F. Decreased bone density and increased phosphaturia in gene-targeted mice lacking functional serum- and glucocorticoid-inducible kinase 3. Kidney international 2011. PMID: 21451460


  52. Finn R, Kovács AD, Pearce DA. Altered sensitivity of cerebellar granule cells to glutamate receptor overactivation in the Cln3(Δex7/8)-knock-in mouse model of juvenile neuronal ceroid lipofuscinosis. Neurochemistry international 2011. PMID: 21315126


  53. Humphreys MH, Ni XP, Pearce D. Cardiovascular effects of melanocortins. Volume 660 of Issue 1. European journal of pharmacology 2011. PMID: 21199648


  54. Pasham V, Rotte A, Bhandaru M, Eichenmüller M, Fröhlich H, Mack AF, Bobbala D, Yang W, Pearce D, Lang F. Regulation of gastric acid secretion by the serum and glucocorticoid inducible kinase isoform SGK3. Journal of gastroenterology 2010. PMID: 21113728


  55. Kovács AD, Saje A, Wong A, Szénási G, Kiricsi P, Szabó E, Cooper JD, Pearce DA. Temporary inhibition of AMPA receptors induces a prolonged improvement of motor performance in a mouse model of juvenile Batten disease. Neuropharmacology 2010. PMID: 20971125


  56. Soundararajan R, Wang J, Melters D, Pearce D. Glucocorticoid-induced Leucine zipper 1 stimulates the epithelial sodium channel by regulating serum- and glucocorticoid-induced kinase 1 stability and subcellular localization. The Journal of biological chemistry 2010. PMID: 20947508


  57. Pao AC, Bhargava A, Di Sole F, Quigley R, Shao X, Wang J, Thomas S, Zhang J, Shi M, Funder JW, Moe OW, Pearce D. Expression and role of serum and glucocorticoid-regulated kinase 2 in the regulation of Na+/H+ exchanger 3 in the mammalian kidney. American journal of physiology. Renal physiology 2010. PMID: 20926631


  58. Zemtsova IM, Heise N, Fröhlich H, Qadri SM, Kucherenko Y, Boini KM, Pearce D, Shumilina E, Lang F. Blunted IgE-mediated activation of mast cells in mice lacking the serum- and glucocorticoid-inducible kinase SGK3. American journal of physiology. Cell physiology 2010. PMID: 20686074


  59. Soundararajan R, Pearce D, Hughey RP, Kleyman TR. Role of epithelial sodium channels and their regulators in hypertension. The Journal of biological chemistry 2010. PMID: 20624922


  60. Rotte A, Pasham V, Bhandaru M, Eichenmüller M, Yang W, Qadri SM, Kempe DS, Puchchakayala G, Pearce D, Birnbaum MJ, Lang F. Regulation of gastric acid secretion by PKB/Akt2. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2010. PMID: 20511715


  61. Hallows KR, Bhalla V, Oyster NM, Wijngaarden MA, Lee JK, Li H, Chandran S, Xia X, Huang Z, Chalkley RJ, Burlingame AL, Pearce D. Phosphopeptide screen uncovers novel phosphorylation sites of Nedd4-2 that potentiate its inhibition of the epithelial Na+ channel. The Journal of biological chemistry 2010. PMID: 20466724


  62. Michael Föller, Daniela S. Kempe, Gulab Siraskar, Henning Fröhlich, Teresa F. Ackermann, Balasaheb Siraskar, Harald Völkl, Martin Judenhofer, Bernd Pichler, Karla F. Leavens, Morris J. Birnbaum, David Pearce, Florian Lang. Akt/PKB-sensitive proximal tubular glucose and phosphate transport. The FASEB Journal 2010. PMID:


  63. Rama Soundararajan, Jian Wang, Daniel Melters, I-Chia Shih, David Pearce. GILZ1: a key molecular determinant of intracellular signaling specificity governing epithelial sodium transport. The FASEB Journal 2010. PMID:


  64. Vivek Bhalla, Nicholas M Oyster, Marjolein Wijngaarden, Jeffrey Lee, Hui Li, Xiaoyu Xia, Zhirong Huang, Robert Chalkley, Alma Burlingame, David Pearce, Ken Hallows. Phosphopeptide Screen Uncovers JNK1 as a Potentiator of Nedd4-2-Mediated Epithelial Na+ Channel Inhibition. The FASEB Journal 2010. PMID:


  65. Lu M, Wang J, Jones KT, Ives HE, Feldman ME, Yao LJ, Shokat KM, Ashrafi K, Pearce D. mTOR complex-2 activates ENaC by phosphorylating SGK1. Journal of the American Society of Nephrology : JASN 2010. PMID: 20338997


  66. Kempe DS, Ackermann TF, Boini KM, Klaus F, Umbach AT, Dërmaku-Sopjani M, Judenhofer MS, Pichler BJ, Capuano P, Stange G, Wagner CA, Birnbaum MJ, Pearce D, Föller M, Lang F. Akt2/PKBbeta-sensitive regulation of renal phosphate transport. Acta physiologica (Oxford, England) 2010. PMID: 20236253


  67. Kempe DS, Siraskar G, Fröhlich H, Umbach AT, Stübs M, Weiss F, Ackermann TF, Völkl H, Birnbaum MJ, Pearce D, Föller M, Lang F. Regulation of renal tubular glucose reabsorption by Akt2/PKBß. American journal of physiology. Renal physiology 2010. PMID: 20164156


  68. Mauro TM, McCormick JA, Wang J, Boini KM, Ray L, Monks B, Birnbaum MJ, Lang F, Pearce D. Akt2 and SGK3 are both determinants of postnatal hair follicle development. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009. PMID: 19433625


  69. Ni XP, van Dijk C, Pearce D, Humphreys MH. Evidence for a noradrenergic mechanism causing hypertension and abnormal glucose metabolism in rats with relative deficiency of gamma-melanocyte-stimulating hormone. Volume 94 of Issue 8. Experimental physiology 2009. PMID: 19429645


  70. Soundararajan R, Melters D, Shih IC, Wang J, Pearce D. Epithelial sodium channel regulated by differential composition of a signaling complex. Proceedings of the National Academy of Sciences of the United States of America 2009. PMID: 19380724


  71. Jones KT, Greer ER, Pearce D, Ashrafi K. Rictor/TORC2 regulates Caenorhabditis elegans fat storage, body size, and development through sgk-1. PLoS biology 2009. PMID: 19260765


  72. Wang J, Knight ZA, Fiedler D, Williams O, Shokat KM, Pearce D. Activity of the p110-alpha subunit of phosphatidylinositol-3-kinase is required for activation of epithelial sodium transport. American journal of physiology. Renal physiology 2008. PMID: 18653476


  73. Soundararajan R, Wang J, Melters D, Pearce D. Differential activities of glucocorticoid-induced leucine zipper protein isoforms. The Journal of biological chemistry 2007. PMID: 17956870


  74. Pao AC, McCormick JA, Li H, Siu J, Govaerts C, Bhalla V, Soundararajan R, Pearce D. NH2 terminus of serum and glucocorticoid-regulated kinase 1 binds to phosphoinositides and is essential for isoform-specific physiological functions. American journal of physiology. Renal physiology 2007. PMID: 17356130


  75. Pearce D, Kleyman TR. Salt, sodium channels, and SGK1. The Journal of clinical investigation 2007. PMID: 17332888


  76. Bhalla V, Oyster NM, Fitch AC, Wijngaarden MA, Neumann D, Schlattner U, Pearce D, Hallows KR. AMP-activated kinase inhibits the epithelial Na+ channel through functional regulation of the ubiquitin ligase Nedd4-2. The Journal of biological chemistry 2006. PMID: 16844684


  77. Bhalla V, Soundararajan R, Pao AC, Li H, Pearce D. Disinhibitory pathways for control of sodium transport: regulation of ENaC by SGK1 and GILZ. American journal of physiology. Renal physiology 2006. PMID: 16720863


  78. Grahammer F, Artunc F, Sandulache D, Rexhepaj R, Friedrich B, Risler T, McCormick JA, Dawson K, Wang J, Pearce D, Wulff P, Kuhl D, Lang F. Renal function of gene-targeted mice lacking both SGK1 and SGK3. American journal of physiology. Regulatory, integrative and comparative physiology 2006. PMID: 16537821


  79. Kenneth R Hallows, Nicholas M Oyster, Vivek Bhalla, David Pearce. Inhibition of the Epithelial Sodium Channel by AMP-Activated Kinase Involves Modulation of Nedd4-2 Activity. The FASEB Journal 2006. PMID:


  80. Lang UE, Wolfer DP, Grahammer F, Strutz-Seebohm N, Seebohm G, Lipp HP, McCormick JA, Hellweg R, Dawson K, Wang J, Pearce D, Lang F. Reduced locomotion in the serum and glucocorticoid inducible kinase 3 knock out mouse. Behavioural brain research 2005. PMID: 16246437


  81. Soundararajan R, Zhang TT, Wang J, Vandewalle A, Pearce D. A novel role for glucocorticoid-induced leucine zipper protein in epithelial sodium channel-mediated sodium transport. The Journal of biological chemistry 2005. PMID: 16216878


  82. Ni XP, Bhargava A, Pearce D, Humphreys MH. Modulation by dietary sodium intake of melanocortin 3 receptor mRNA and protein abundance in the rat kidney. Volume 290 of Issue 3. American journal of physiology. Regulatory, integrative and comparative physiology 2005. PMID: 16195498


  83. Bhalla V, Daidié D, Li H, Pao AC, LaGrange LP, Wang J, Vandewalle A, Stockand JD, Staub O, Pearce D. Serum- and glucocorticoid-regulated kinase 1 regulates ubiquitin ligase neural precursor cell-expressed, developmentally down-regulated protein 4-2 by inducing interaction with 14-3-3. Molecular endocrinology (Baltimore, Md.) 2005. PMID: 16099816


  84. Sandu C, Rexhepaj R, Grahammer F, McCormick JA, Henke G, Palmada M, Nammi S, Lang U, Metzger M, Just L, Skutella T, Dawson K, Wang J, Pearce D, Lang F. Decreased intestinal glucose transport in the sgk3-knockout mouse. Pflugers Archiv : European journal of physiology 2005. PMID: 15971077


  85. McCormick JA, Bhalla V, Pao AC, Pearce D. SGK1: a rapid aldosterone-induced regulator of renal sodium reabsorption. Physiology (Bethesda, Md.) 2005. PMID: 15772302


  86. Strutz-Seebohm N, Seebohm G, Mack AF, Wagner HJ, Just L, Skutella T, Lang UE, Henke G, Striegel M, Hollmann M, Rouach N, Nicoll RA, McCormick JA, Wang J, Pearce D, Lang F. Regulation of GluR1 abundance in murine hippocampal neurones by serum- and glucocorticoid-inducible kinase 3. The Journal of physiology 2005. PMID: 15774536


  87. Chen X, Moerschell RP, Pearce DA, Ramanan DD, Sherman F. Enhanced mitochondrial degradation of yeast cytochrome c with amphipathic structures. Current genetics 2004. PMID: 15605252


  88. Meijer OC, Kalkhoven E, van der Laan S, Steenbergen PJ, Houtman SH, Dijkmans TF, Pearce D, de Kloet ER. Steroid receptor coactivator-1 splice variants differentially affect corticosteroid receptor signaling. Endocrinology 2004. PMID: 15564339


  89. McCormick JA, Feng Y, Dawson K, Behne MJ, Yu B, Wang J, Wyatt AW, Henke G, Grahammer F, Mauro TM, Lang F, Pearce D. Targeted disruption of the protein kinase SGK3/CISK impairs postnatal hair follicle development. Molecular biology of the cell 2004. PMID: 15240817


  90. Bhargava A, Dallman MF, Pearce D, Choi S. Long double-stranded RNA-mediated RNA interference as a tool to achieve site-specific silencing of hypothalamic neuropeptides. Brain research. Brain research protocols 2004. PMID: 15171994


  91. Bhargava A, Pearce D. Mechanisms of mineralocorticoid action: determinants of receptor specificity and actions of regulated gene products. Trends in endocrinology and metabolism: TEM 2004. PMID: 15109612


  92. Bhargava A, Wang J, Pearce D. Regulation of epithelial ion transport by aldosterone through changes in gene expression. Molecular and cellular endocrinology 2004. PMID: 15134817


  93. Chen S, McCormick JA, Prabaker K, Wang J, Pearce D, Gardner DG. Sgk1 mediates osmotic induction of NPR-A gene in rat inner medullary collecting duct cells. Volume 43 of Issue 4. Hypertension (Dallas, Tex. : 1979) 2004. PMID: 15007040


  94. Adams M, Meijer OC, Wang J, Bhargava A, Pearce D. Homodimerization of the glucocorticoid receptor is not essential for response element binding: activation of the phenylethanolamine N-methyltransferase gene by dimerization-defective mutants. Molecular endocrinology (Baltimore, Md.) 2003. PMID: 12933902


  95. Greenwood AK, Butler PC, White RB, DeMarco U, Pearce D, Fernald RD. Multiple corticosteroid receptors in a teleost fish: distinct sequences, expression patterns, and transcriptional activities. Endocrinology 2003. PMID: 12959971


  96. Ni XP, Pearce D, Butler AA, Cone RD, Humphreys MH. Genetic disruption of gamma-melanocyte-stimulating hormone signaling leads to salt-sensitive hypertension in the mouse. Volume 111 of Issue 8. The Journal of clinical investigation 2003. PMID: 12697744


  97. Friedrich B, Feng Y, Cohen P, Risler T, Vandewalle A, Bröer S, Wang J, Pearce D, Lang F. The serine/threonine kinases SGK2 and SGK3 are potent stimulators of the epithelial Na+ channel alpha,beta,gamma-ENaC. Pflugers Archiv : European journal of physiology 2003. PMID: 12632189


  98. Pearce D, Bhargava A, Cole TJ. Aldosterone: its receptor, target genes, and actions. Vitamins and hormones 2003. PMID: 12852252


  99. Pearce D. SGK1 regulation of epithelial sodium transport. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2003. PMID: 12649598


  100. Yun CC, Palmada M, Embark HM, Fedorenko O, Feng Y, Henke G, Setiawan I, Boehmer C, Weinman EJ, Sandrasagra S, Korbmacher C, Cohen P, Pearce D, Lang F. The serum and glucocorticoid-inducible kinase SGK1 and the Na+/H+ exchange regulating factor NHERF2 synergize to stimulate the renal outer medullary K+ channel ROMK1. Journal of the American Society of Nephrology : JASN 2002. PMID: 12444200


  101. Edinger RS, Watkins SC, Pearce D, Johnson JP. Effect of immunosuppressive agents on glucocorticoid receptor function in A6 cells. American journal of physiology. Renal physiology 2002. PMID: 12110508


  102. Bhargava A, Mathias RS, McCormick JA, Dallman MF, Pearce D. Glucocorticoids prolong Ca(2+) transients in hippocampal-derived H19-7 neurons by repressing the plasma membrane Ca(2+)-ATPase-1. Molecular endocrinology (Baltimore, Md.) 2002. PMID: 12089356


  103. Rozansky DJ, Wang J, Doan N, Purdy T, Faulk T, Bhargava A, Dawson K, Pearce D. Hypotonic induction of SGK1 and Na+ transport in A6 cells. American journal of physiology. Renal physiology 2002. PMID: 12060592


  104. Debonneville C, Flores SY, Kamynina E, Plant PJ, Tauxe C, Thomas MA, Münster C, Chraïbi A, Pratt JH, Horisberger JD, Pearce D, Loffing J, Staub O. Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+) channel cell surface expression. The EMBO journal 2001. PMID: 11742982


  105. Pearce D, Náray-Fejes-Tóth A, Fejes-Tóth G. Determinants of subnuclear organization of mineralocorticoid receptor characterized through analysis of wild type and mutant receptors. The Journal of biological chemistry 2001. PMID: 11677231


  106. Pearce D. The role of SGK1 in hormone-regulated sodium transport. Trends in endocrinology and metabolism: TEM 2001. PMID: 11551807


  107. Loffing J, Zecevic M, Féraille E, Kaissling B, Asher C, Rossier BC, Firestone GL, Pearce D, Verrey F. Aldosterone induces rapid apical translocation of ENaC in early portion of renal collecting system: possible role of SGK. American journal of physiology. Renal physiology 2001. PMID: 11249859


  108. Aditi Bhargava, Meryl J. Fullerton, Kathy Myles, Timothy M. Purdy, John W. Funder, David Pearce, Timothy J. Cole. The Serum- and Glucocorticoid-Induced Kinase Is a Physiological Mediator of Aldosterone Action*. Endocrinology 2001. PMID:


  109. Bhargava A, Fullerton MJ, Myles K, Purdy TM, Funder JW, Pearce D, Cole TJ. The serum- and glucocorticoid-induced kinase is a physiological mediator of aldosterone action. Endocrinology 2001. PMID: 11250940


  110. Wang J, Barbry P, Maiyar AC, Rozansky DJ, Bhargava A, Leong M, Firestone GL, Pearce D. SGK integrates insulin and mineralocorticoid regulation of epithelial sodium transport. American journal of physiology. Renal physiology 2001. PMID: 11208606


  111. Sherman MP, de Noronha CM, Pearce D, Greene WC. Human immunodeficiency virus type 1 Vpr contains two leucine-rich helices that mediate glucocorticoid receptor coactivation independently of its effects on G(2) cell cycle arrest. Journal of virology 2000. PMID: 10933727


  112. Iñiguez-Lluhí JA, Pearce D. A common motif within the negative regulatory regions of multiple factors inhibits their transcriptional synergy. Molecular and cellular biology 2000. PMID: 10913186


  113. Bhargava A, Meijer OC, Dallman MF, Pearce D. Plasma membrane calcium pump isoform 1 gene expression is repressed by corticosterone and stress in rat hippocampus. The Journal of neuroscience : the official journal of the Society for Neuroscience 2000. PMID: 10777776


  114. Verrey F, Pearce D, Pfeiffer R, Spindler B, Mastroberardino L, Summa V, Zecevic M. Pleiotropic action of aldosterone in epithelia mediated by transcription and post-transcription mechanisms. Kidney international 2000. PMID: 10760054


  115. Pearce D, Verrey F, Chen SY, Mastroberardino L, Meijer OC, Wang J, Bhargava A. Role of SGK in mineralocorticoid-regulated sodium transport. Kidney international 2000. PMID: 10760055


  116. Meijer OC, Williamson A, Dallman MF, Pearce D. Transcriptional repression of the 5-HT1A receptor promoter by corticosterone via mineralocorticoid receptors depends on the cellular context. Journal of neuroendocrinology 2000. PMID: 10718920


  117. Wissink S, Meijer O, Pearce D, van Der Burg B, van Der Saag PT. Regulation of the rat serotonin-1A receptor gene by corticosteroids. The Journal of biological chemistry 2000. PMID: 10625680


  118. Pearce DA, Sherman F. Toxicity of copper, cobalt, and nickel salts is dependent on histidine metabolism in the yeast Saccharomyces cerevisiae. Journal of bacteriology 1999. PMID: 10438744


  119. Rogatsky I, Hittelman AB, Pearce D, Garabedian MJ. Distinct glucocorticoid receptor transcriptional regulatory surfaces mediate the cytotoxic and cytostatic effects of glucocorticoids. Molecular and cellular biology 1999. PMID: 10373553


  120. Chen SY, Bhargava A, Mastroberardino L, Meijer OC, Wang J, Buse P, Firestone GL, Verrey F, Pearce D. Epithelial sodium channel regulated by aldosterone-induced protein sgk. Proceedings of the National Academy of Sciences of the United States of America 1999. PMID: 10051674


  121. Pearce D, Matsui W, Miner JN, Yamamoto KR. Glucocorticoid receptor transcriptional activity determined by spacing of receptor and nonreceptor DNA sites. The Journal of biological chemistry 1998. PMID: 9804760


  122. Fejes-Tóth G, Pearce D, Náray-Fejes-Tóth A. Subcellular localization of mineralocorticoid receptors in living cells: effects of receptor agonists and antagonists. Proceedings of the National Academy of Sciences of the United States of America 1998. PMID: 9501200


  123. Chen SY, Wang J, Liu W, Pearce D. Aldosterone responsiveness of A6 cells is restored by cloned rat mineralocorticoid receptor. The American journal of physiology 1998. PMID: 9458711


  124. Chen Sy, Wang J, Yu Gq, Liu W, Pearce D. Androgen and glucocorticoid receptor heterodimer formation. A possible mechanism for mutual inhibition of transcriptional activity. The Journal of biological chemistry 1997. PMID: 9162033


  125. Page MD, Pearce DA, Norris HA, Ferguson SJ. The Paracoccus denitrificans ccmA, B and C genes: cloning and sequencing, and analysis of the potential of their products to form a haem or apo- c-type cytochrome transporter. Microbiology (Reading, England) 1997. PMID: 9043133


  126. Liu W, Wang J, Yu G, Pearce D. Steroid receptor transcriptional synergy is potentiated by disruption of the DNA-binding domain dimer interface. Molecular endocrinology (Baltimore, Md.) 1996. PMID: 8923466


  127. Liu W, Wang J, Sauter NK, Pearce D. Steroid receptor heterodimerization demonstrated in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America 1995. PMID: 8618925


  128. Pearce D. A mechanistic basis for distinct mineralocorticoid and glucocorticoid receptor transcriptional specificities. Steroids 1994. PMID: 8191546


  129. Pearce D, Yamamoto KR. Mineralocorticoid and glucocorticoid receptor activities distinguished by nonreceptor factors at a composite response element. Science (New York, N.Y.) 1993. PMID: 8382376


  130. Krapf R, Pearce D, Lynch C, Xi XP, Reudelhuber TL, Pouysségur J, Rector FC. Expression of rat renal Na/H antiporter mRNA levels in response to respiratory and metabolic acidosis. The Journal of clinical investigation 1991. PMID: 1846882


  131. Pearce D, Verkman AS. NaCl reflection coefficients in proximal tubule apical and basolateral membrane vesicles. Measurement by induced osmosis and solvent drag. Biophysical journal 1989. PMID: 2765660


  132. Chen PY, Pearce D, Verkman AS. Membrane water and solute permeability determined quantitatively by self-quenching of an entrapped fluorophore. Biochemistry 1988. PMID: 3179272