Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
Authors: Leibovici L, Paul M, Doernberg SB
PloS one
Authors: Khandu L, Kinley K, Norbu YC, Tobgay T, Tsheten T, Gyeltshen T, Choden S, McFarland W
Volume 378 of Issue 6615 | Science (New York, N.Y.)
Authors: Richards CM, Jabs S, Qiao W, Varanese LD, Schweizer M, Mosen PR, Riley NM, Klüssendorf M, Zengel JR, Flynn RA, Rustagi A, Widen JC, Peters CE, Ooi YS, Xie X, Shi PY, Bartenschlager R, Puschnik AS, Bogyo M, Bertozzi CR, Blish CA, Winter D, Nagamine CM, Braulke T, Carette JE
Lysosomes are key degradative compartments of the cell. Transport to lysosomes relies on GlcNAc-1-phosphotransferase-mediated tagging of soluble enzymes with mannose 6-phosphate (M6P). GlcNAc-1-phosphotransferase deficiency leads to the severe lysosomal storage disorder mucolipidosis II (MLII). Several viruses require lysosomal cathepsins to cleave structural proteins and thus depend on functional GlcNAc-1-phosphotransferase. We used genome-scale CRISPR screens to identify lysosomal enzyme trafficking factor (LYSET, also named TMEM251) as essential for infection by cathepsin-dependent viruses including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). LYSET deficiency resulted in global loss of M6P tagging and mislocalization of GlcNAc-1-phosphotransferase from the Golgi complex to lysosomes. knockout mice exhibited MLII-like phenotypes, and human pathogenic LYSET alleles failed to restore lysosomal sorting defects. Thus, LYSET is required for correct functioning of the M6P trafficking machinery and mutations in LYSET can explain the phenotype of the associated disorder.
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Annals of oncology : official journal of the European Society for Medical Oncology
Authors: Seufferlein T, Uhl W, Kornmann M, Alg?l H, Friess H, K?nig A, Ghadimi M, Gallmeier E, Bartsch DK, Lutz MP, Metzger R, Wille K, Gerdes B, Schimanski CC, Graupe F, Kunzmann V, Klein I, Geissler M, Staib L, Waldschmidt D, Bruns C, Wittel U, Fichtner-Feigl S, Daum S, Hinke A, Blome L, Tannapfel A, Kleger A, Berger AW, Kestler AMR, Schuhbaur JS, Perkhofer L, Tempero M, Reinacher-Schick AC, Ettrich TJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Authors: Eastell R, Vittinghoff E, Lui LY, Ewing SK, Schwartz AV, Bauer DC, Black DM, Bouxsein ML
Scientific Reports
Authors: Elahi M, Talreja J, Steinbauer B, Koth LL, Samavati L
Preventive medicine
Authors: Gafni T, Gabriel KP, Shuval K, Yaffe K, Sidney S, Weinstein G
Journal of the American Medical Informatics Association : JAMIA
Authors: Alexander J, Beatty A
Transgender Health
Authors: Bindman J, Ngo A, Zamudio-Haas S, Sevelius J
Journal of diabetes science and technology
Authors: Huang J, Yeung AM, Eiland LA, Huang ES, Raymond JK, Klonoff DC
