Publications

BACKGROUND

Experimental human stem cell transplantation to the heart has begun, but the mechanisms underlying benefits seen in preclinical models, both at the site of cell injection and at more distant regions, remain uncertain. We hypothesize that these benefits can be best understood first at the level of key intracellular signaling cascades in the host myocardium, which can be responsible for functional and structural preservation of the heart.

STUDY DESIGN

Western blot and ELISA were used to assess key pathways that regulate cardiac myocyte survival and hypertrophy in both the infarct/borderzone and remote myocardium of C57/B6 mouse hearts subjected to coronary artery ligation, with subsequent injection of either vehicle or bone marrow-derived adult mesenchymal stem cells (MSC).

RESULTS

Improved left ventricular function with MSC transplantation was associated with a relative preservation of Akt phosphorylation (activation) and of phosphorylation of downstream mediators of cell survival and hypertrophy. There was no substantial difference in activation of mitogen-activated protein kinase p38, and activation of the antiapoptotic mitogen-activated protein kinase extracellular signal-regulated kinase was lower at 1 week after MSC treatment, but rose beyond controls by week 2. Similar changes were observed in both the infarct/borderzone and the remote myocardium.

CONCLUSION

Stem cell transplantation in the post-MI murine myocardium is associated with preservation of Akt signaling. Together with a possible later increase in extracellular signal-regulated kinase activation, this signaling change might be responsible for cardioprotection. Additional focused investigation might identify elements in transplantation regimens that optimize this mechanism of benefit, and that can increase the likelihood of human clinical success.

BACKGROUND

Lack of awareness may be a significant barrier to participation by low- and middle-income seniors in pharmaceutical cost-assistance programs.

OBJECTIVE

The goal of this study was to determine whether older adults' awareness of 2 major state and federal pharmaceutical cost-assistance programs was associated with the seniors' ability to access and process information about assistance programs.

METHODS

Data were gathered from a cross-sectional study of independently living, English- or Spanish-speaking adults aged > or =60 years. Participants were interviewed in 30 community-based settings (19 apartment complexes and 11 senior centers) in New York, New York. The analysis focused on adults aged > or =65 years who lacked Medicaid coverage. Multivariable logistic regression was used to model program awareness as a function of information access (family/social support, attendance at senior or community centers and places of worship, viewing of live health insurance presentations, instrumental activities of daily living, site of medical care, computer use, and having a proxy decision maker for health insurance matters) and information-processing ability (education level, English proficiency, health literacy, and cognitive function). The main outcome measure was awareness of New York's state pharmaceutical assistance program (Elderly Pharmaceutical Insurance Coverage [EPIC]]) and the federal Medicare Part D low-income subsidy program (Extra Help).

RESULTS

A total of 269 patients were enrolled (mean [SD] age, 76.9 [7.5] years; 32.0% male; 39.9% white). Awareness of the programs differed widely: 77.3%) knew of EPIC! and 22.3% knew of Extra Help. In multivariable analysis, study participants were more likely to have heard of the EPIC program if they had attended a live presentation about health insurance issues (adjusted odds ratio [AOR], 3.40; 95% CI, 1.20-9.61) and less likely if they received care in a clinic (AOR, 0.45; 95% CI, 0.23-0.92). Awareness of Extra Help in the multivariable models was more likely among study participants who had viewed a live health insurance presentation (AOR, 3.35; 95% CI, 1.55-7.24) and less likely for those with inadequate health literacy (AOR, 0.15; 95% CI, 0.03-0.74).

CONCLUSIONS

Viewing of live health insurance presentations and adequate health literacy were associated with greater awareness of important pharmaceutical cost-assistance programs in this study in low-income, elderly individuals. The findings suggest that use of live presentations, in addition to health literacy materials and messages, may be important strategies in promoting knowledge of and enrollment in state and federal pharmaceutical cost-assistance programs for low-income seniors.

BACKGROUND

The goal of this study was to develop an accurate and reliable supine plantar pressure measurement apparatus that could potentially be used in the operating room to guide operative decision making. It was hypothesized that plantar pressures would be similar in supine and vertical posture trials.

MATERIALS AND METHODS

A supine plantar pressure system was developed using the Pliance 32 sensor array (Novel, Munich, Germany). Accuracy was tested with a standardized manometer and the Trublu calibration system (Novel). Next, bilateral feet of 10 healthy, asymptomatic patients were tested in the apparatus by two separate investigators. Intraclass correlation coefficients (ICC) were calculated to determine intrarater and interrater reliability for parameters of average mean pressure (AMP), peak pressure (PP) and percentage of total force (PF). Finally, plantar pressures were tested in vertical posture and compared to supine parameters.

RESULTS

The sensor was both linear and accurate. The ICC values demonstrated that the sensor was reliable for AMP (0.66 to 0.93), PP (0.75 to 0.94), and PF (0.69 to 0.97). In general, AMP, PP, and PF values were significantly different at most anatomical regions for supine and vertical posture trials (p < 0.0001).

CONCLUSION

This intraoperative pressure measurement system provided an accurate, linear, and reliable method to measure plantar pressure parameters in the supine subject. These measures were similar in magnitude to vertical posture, but were statistically different.

CLINICAL RELEVANCE

This intraoperative plantar pressure system could guide operative reconstruction of foot deformities whose outcome depends on the distribution of pressures across the plantar foot.