Inquiry : a journal of medical care organization, provision and financing
Authors: Ko M, Newcomer RJ, Harrington C, Hulett D, Kang T, Bindman AB
Volume 1768 | Methods in molecular biology (Clifton, N.J.)
Authors: Miyaoka Y, Mayerl SJ, Chan AH, Conklin BR
Genome editing holds great promise for experimental biology and potential clinical use. To successfully utilize genome editing, it is critical to sensitively detect and quantify its outcomes: homology-directed repair (HDR) and nonhomologous end joining (NHEJ). This has been difficult at endogenous gene loci and instead is frequently done using artificial reporter systems. Here, we describe a droplet digital PCR (ddPCR)-based method to simultaneously measure HDR and NHEJ at endogenous gene loci. This highly sensitive and quantitative method may significantly contribute to a better understanding of DNA repair mechanisms underlying genome editing and to the improvement of genome editing technology by allowing for efficient and systematic testing of many genome editing conditions in parallel.
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Journal of hospital medicine
Authors: Auerbach AD
European heart journal cardiovascular Imaging
Authors: Lu DY, Haileselassie B, Ventoulis I, Liu HY, Liang HY, Pozios I, Canepa M, Phillip S, Abraham MR, Abraham T
The American journal of managed care
Authors: Chang E, Blondon K, Lyles CR, Jordan L, Ralston JD
Journal of the American Board of Family Medicine : JABFM
Authors: Kowalski CP, McQuillan DB, Chawla N, Lyles C, Altschuler A, Uratsu CS, Bayliss EA, Heisler M, Grant RW
The Permanente journal
Authors: Stenmark SH, Steiner JF, Marpadga S, Debor M, Underhill K, Seligman H
JAMA internal medicine
Authors: Moran AE, Pletcher MJ, Bibbins-Domingo K
American journal of preventive medicine
Authors: Wolff TA, Krist AH, LeFevre M, Jonas DE, Harris RP, Siu A, Owens DK, Gillman MW, Ebell MH, Herzstein J, Chou R, Whitlock E, Bibbins-Domingo K
American journal of preventive medicine
Authors: Ngo-Metzger Q, Moyer V, Grossman D, Ebell M, Woo M, Miller T, Brummer T, Chowdhury J, Kato E, Siu A, Phillips W, Davidson K, Phipps M, Bibbins-Domingo K