Sharof Tugizov, PhD

Adjunct Professor

A goal of my laboratory is to understand the molecular mechanisms of the interactions of human viruses, including human immunodeficiency virus and herpes viruses with oropharyngeal and anogenital mucosal epithelia. Specifically, we investigate the molecular mechanisms of transepithelial transmission of these viruses through the mucosal epithelium. Understanding viral transmission via the mucosal epithelium is important for preventing the initial steps of viral infection.

Education
Postdoctoral Studies, - Graduate Division, University of California, San Francisco
Publications

  1. Cai X, Padilla NT, Rosbe K, Tugizov SM. Breast milk induces the differentiation of monocytes into macrophages, promoting human cytomegalovirus infection. Journal of virology 2024. PMID: 39194236


  2. Tugizov SM. Molecular Pathogenesis of Human Immunodeficiency Virus-Associated Disease of Oropharyngeal Mucosal Epithelium. Biomedicines 2023. PMID: 37239115


  3. Walston JJ, Hayman IR, Gore M, Ferguson M, Temple RM, Liao J, Alam S, Meyers C, Tugizov SM, Hutt-Fletcher L, Sample CE. The Epstein-Barr Virus Glycoprotein BDLF2 Is Essential for Efficient Viral Spread in Stratified Epithelium. Journal of virology 2023. PMID: 36688650


  4. Lien K, Mayer W, Herrera R, Padilla NT, Cai X, Lin V, Pholcharoenchit R, Palefsky J, Tugizov SM. HIV-1 Proteins gp120 and Tat Promote Epithelial-Mesenchymal Transition and Invasiveness of HPV-Positive and HPV-Negative Neoplastic Genital and Oral Epithelial Cells. Microbiology spectrum 2022. PMID: 36314970


  5. Herrera R, Rosbe K, Tugizov SM. Inactivation of HIV-1 in Polarized Infant Tonsil Epithelial Cells by Human Beta-Defensins 2 and 3 Tagged with the Protein Transduction Domain of HIV-1 Tat. 2021. PMID: 34696473


  6. Sufiawati I, Herrera R, Mayer W, Cai X, Borkakoti J, Lin V, Rosbe K, Tugizov SM. Human immunodeficiency virus and human cytomegalovirus coinfection of infant tonsil epithelium may synergistically promote both HIV-1 and HCMV spread and infection. Journal of virology 2021. PMID: 34232730


  7. Tugizov S. Virus-associated disruption of mucosal epithelial tight junctions and its role in viral transmission and spread. Tissue barriers 2021. PMID: 34241579


  8. Tugizov SM. Human immunodeficiency virus interaction with oral and genital mucosal epithelia may lead to epithelial-mesenchymal transition and sequestration of virions in the endosomal compartments. Oral diseases 2020. PMID: 32862547


  9. Weinberg A, Tugizov S, Pandiyan P, Jin G, Rakshit S, Vyakarnam A, Naglik JR. Innate immune mechanisms to oral pathogens in oral mucosa of HIV-infected individuals. Oral diseases 2020. PMID: 32862519


  10. Lien K, Mayer W, Herrera R, Rosbe K, Tugizov SM. HIV-1 proteins gp120 and tat induce the epithelial-mesenchymal transition in oral and genital mucosal epithelial cells. PloS one 2019. PMID: 31869348


  11. Walhart T, Isaacson-Wechsler E, Ang KH, Arkin M, Tugizov S, Palefsky JM. A Cell-Based Renilla Luminescence Reporter Plasmid Assay for High-Throughput Screening to Identify Novel FDA-Approved Drug Inhibitors of HPV-16 Infection. SLAS discovery : advancing life sciences R & D 2019. PMID: 31361520


  12. Wechsler EI, Tugizov S, Herrera R, Costa MD, Palefsky JM. Erratum: E5 can be expressed in anal cancer and leads to epidermal growth factor receptor-induced invasion in a human papillomavirus 16-transformed anal epithelial cell line. The Journal of general virology 2018. PMID: 29966543


  13. Sufiawati I, Tugizov SM. HIV-induced matrix metalloproteinase-9 activation through mitogen-activated protein kinase signalling promotes HSV-1 cell-to-cell spread in oral epithelial cells. The Journal of general virology 2018. PMID: 29775175


  14. Wechsler EI, Tugizov S, Herrera R, Da Costa M, Palefsky JM. E5 can be expressed in anal cancer and leads to epidermal growth factor receptor-induced invasion in a human papillomavirus 16-transformed anal epithelial cell line. The Journal of general virology 2018. PMID: 29624161


  15. Yasen A, Herrera R, Rosbe K, Lien K, Tugizov SM. HIV internalization into oral and genital epithelial cells by endocytosis and macropinocytosis leads to viral sequestration in the vesicles. Virology 2017. PMID: 29277006


  16. Yasen A, Herrera R, Rosbe K, Lien K, Tugizov SM. Release of HIV-1 sequestered in the vesicles of oral and genital mucosal epithelial cells by epithelial-lymphocyte interaction. PLoS pathogens 2017. PMID: 28241053


  17. Haga T, Efird JT, Tugizov S, Palefsky JM. Increased TNF-alpha and sTNFR2 levels are associated with high-grade anal squamous intraepithelial lesions in HIV-positive patients with low CD4 level. Papillomavirus research (Amsterdam, Netherlands) 2016. PMID: 28720441


  18. Tugizov S. Human immunodeficiency virus-associated disruption of mucosal barriers and its role in HIV transmission and pathogenesis of HIV/AIDS disease. Tissue barriers 2016. PMID: 27583187


  19. Herrera R, Morris M, Rosbe K, Feng Z, Weinberg A, Tugizov S. Human beta-defensins 2 and -3 cointernalize with human immunodeficiency virus via heparan sulfate proteoglycans and reduce infectivity of intracellular virions in tonsil epithelial cells. Virology 2015. PMID: 26539799


  20. Sufiawati I, Tugizov SM. HIV-associated disruption of tight and adherens junctions of oral epithelial cells facilitates HSV-1 infection and spread. PloS one 2014. PMID: 24586397


  21. Tugizov SM, Herrera R, Chin-Hong P, Veluppillai P, Greenspan D, Michael Berry J, Pilcher CD, Shiboski CH, Jay N, Rubin M, Chein A, Palefsky JM. HIV-associated disruption of mucosal epithelium facilitates paracellular penetration by human papillomavirus. Virology 2013. PMID: 24074602


  22. Tugizov SM, Herrera R, Palefsky JM. Epstein-Barr virus transcytosis through polarized oral epithelial cells. Journal of virology 2013. PMID: 23698302


  23. . 46. HIV-induced epithelial–mesenchymal transition in mucosal epithelium facilitates HPV paracellular penetration. 2013. PMID:


  24. . 48. Cidofovir treatment prevents invasion of invasive HPV-16-positive anal keratinocytes. 2013. PMID:


  25. . Track A Basic Science. 2012. PMID:


  26. Tugizov SM, Herrera R, Veluppillai P, Greenspan D, Soros V, Greene WC, Levy JA, Palefsky JM. Differential transmission of HIV traversing fetal oral/intestinal epithelia and adult oral epithelia. Volume 86 of Issue 5. Journal of virology 2011. PMID: 22205732


  27. Challacombe SJ, Fidel PL, Tugizov S, Tao L, Wahl SM. HIV infection and specific mucosal immunity: workshop 4B. Advances in dental research 2011. PMID: 21441496


  28. Weinberg A, Naglik JR, Kohli A, Tugizov SM, Fidel PL, Liu Y, Herzberg M. Innate immunity including epithelial and nonspecific host factors: workshop 1B. Advances in dental research 2011. PMID: 21441493


  29. Tugizov SM, Webster-Cyriaque JY, Syrianen S, Chattopadyay A, Sroussi H, Zhang L, Kaushal A. Mechanisms of viral infections associated with HIV: workshop 2B. Advances in dental research 2011. PMID: 21441494


  30. Tugizov SM, Herrera R, Veluppillai P, Greenspan D, Soros V, Greene WC, Levy JA, Palefsky JM. HIV is inactivated after transepithelial migration via adult oral epithelial cells but not fetal epithelial cells. Volume 409 of Issue 2. Virology 2010. PMID: 21056450


  31. Dorner M, Zucol F, Alessi D, Haerle SK, Bossart W, Weber M, Byland R, Bernasconi M, Berger C, Tugizov S, Speck RF, Nadal D. beta1 integrin expression increases susceptibility of memory B cells to Epstein-Barr virus infection. Journal of virology 2010. PMID: 20427540


  32. Xiao J, Palefsky JM, Herrera R, Sunshine C, Tugizov SM. EBV-positive human sera contain antibodies against the EBV BMRF-2 protein. Virology 2009. PMID: 19698968


  33. Xiao J, Palefsky JM, Herrera R, Berline J, Tugizov SM. EBV BMRF-2 facilitates cell-to-cell spread of virus within polarized oral epithelial cells. Virology 2009. PMID: 19394065


  34. Haga T, Uchide N, Tugizov S, Palefsky JM. Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation. Apoptosis : an international journal on programmed cell death 2008. PMID: 17906929


  35. Xiao J, Palefsky JM, Herrera R, Berline J, Tugizov SM. The Epstein-Barr virus BMRF-2 protein facilitates virus attachment to oral epithelial cells. Virology 2007. PMID: 17945327


  36. Tugizov S, Herrera R, Veluppillai P, Greenspan J, Greenspan D, Palefsky JM. Epstein-Barr virus (EBV)-infected monocytes facilitate dissemination of EBV within the oral mucosal epithelium. Journal of virology 2007. PMID: 17376918


  37. Xiao J, Palefsky JM, Herrera R, Tugizov SM. Characterization of the Epstein-Barr virus glycoprotein BMRF-2. Virology 2006. PMID: 17081581


  38. Tugizov S, Berline J, Herrera R, Penaranda ME, Nakagawa M, Palefsky J. Inhibition of human papillomavirus type 16 E7 phosphorylation by the S100 MRP-8/14 protein complex. Journal of virology 2005. PMID: 15613338


  39. . Erratum: Epstein-Barr virus infection of polarized tongue and nasopharyngeal epithelial cells. 2003. PMID:


  40. . Erratum: Epstein-Barr virus infection of polarized tongue and nasopharyngeal epithelial cells. 2003. PMID:


  41. Tugizov SM, Berline JW, Palefsky JM. Epstein-Barr virus infection of polarized tongue and nasopharyngeal epithelial cells. Nature medicine 2003. PMID: 12592401


  42. Tugizov S, Maidji E, Xiao J, Pereira L. An acidic cluster in the cytosolic domain of human cytomegalovirus glycoprotein B is a signal for endocytosis from the plasma membrane. Journal of virology 1999. PMID: 10482621


  43. Tugizov S, Maidji E, Xiao J, Zheng Z, Pereira L. Human cytomegalovirus glycoprotein B contains autonomous determinants for vectorial targeting to apical membranes of polarized epithelial cells. Journal of virology 1998. PMID: 9696834


  44. Maidji E, Tugizov S, Abenes G, Jones T, Pereira L. A novel human cytomegalovirus glycoprotein, gpUS9, which promotes cell-to-cell spread in polarized epithelial cells, colocalizes with the cytoskeletal proteins E-cadherin and F-actin. Journal of virology 1998. PMID: 9621030


  45. Navarro D, Lennette E, Tugizov S, Pereira L. Humoral immune response to functional regions of human cytomegalovirus glycoprotein B. Journal of medical virology 1997. PMID: 9260696


  46. Maidji E, Tugizov S, Jones T, Zheng Z, Pereira L. Accessory human cytomegalovirus glycoprotein US9 in the unique short component of the viral genome promotes cell-to-cell transmission of virus in polarized epithelial cells. Journal of virology 1996. PMID: 8970961


  47. Zheng Z, Maidji E, Tugizov S, Pereira L. Mutations in the carboxyl-terminal hydrophobic sequence of human cytomegalovirus glycoprotein B alter transport and protein chaperone binding. Journal of virology 1996. PMID: 8892927


  48. Tugizov S, Maidji E, Pereira L. Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells. The Journal of general virology 1996. PMID: 8558129


  49. Tugizov S, Wang Y, Qadri I, Navarro D, Maidji E, Pereira L. Mutated forms of human cytomegalovirus glycoprotein B are impaired in inducing syncytium formation. Virology 1995. PMID: 7778290


  50. Ramakrishnan M, Tugizov S, Pereira L, Lee AS. Conformation-defective herpes simplex virus 1 glycoprotein B activates the promoter of the grp94 gene that codes for the 94-kD stress protein in the endoplasmic reticulum. DNA and cell biology 1995. PMID: 7748487


  51. Pereira L, Maidji E, Tugizov S, Jones T. Deletion mutants in human cytomegalovirus glycoprotein US9 are impaired in cell-cell transmission and in altering tight junctions of polarized human retinal pigment epithelial cells. Scandinavian journal of infectious diseases. Supplementum 1995. PMID: 8668948


  52. Tugizov S, Navarro D, Paz P, Wang Y, Qadri I, Pereira L. Function of human cytomegalovirus glycoprotein B: syncytium formation in cells constitutively expressing gB is blocked by virus-neutralizing antibodies. Virology 1994. PMID: 8184537


  53. Tugizov SM, Savchenkova IP, Grabovskaya IL, Makarova NE, Eraizer TL, Revazova EP, Kushch AA. Changes in expression of surface and core antigens of hepatitis B virus in different mutant clones of hepatoma PLC-PRF-5 cells. Virus research 1993. PMID: 8249446


  54. Navarro D, Paz P, Tugizov S, Topp K, La Vail J, Pereira L. Glycoprotein B of human cytomegalovirus promotes virion penetration into cells, transmission of infection from cell to cell, and fusion of infected cells. Virology 1993. PMID: 7692667


  55. Grabovskaya IL, Tugizov SM, Glukhova LA, Kushch AA. Cytogenetic analysis of human hepatocarcinoma cell line PLC-PRF-5 and its mutant clones with different degrees of cell differentiation. Cancer genetics and cytogenetics 1993. PMID: 8384075


  56. Tugizov ShM, Savchenkova IP, Grabovskaia IL, Kushch AA. [Changes in the gene expression of the hepatitis B virus in PLC-PRF-5 cells of human hepatocarcinoma during their acquisition of drug resistance]. Voprosy virusologii 1992. PMID: 1337803


  57. Kushch AA, Tugizov ShM, Terletskaia EN, Posevaia TA, Nikitina AA, L'vov ND, Barinskii IF. [Human hybridoma neutralizing antibodies to the herpes simplex virus]. Voprosy virusologii 1992. PMID: 1337804


  58. Shumai EP, Vorob'ev SM, Makarova NE, Tugizov ShM, Zverev VV, Kushch AA. [The immunoenzyme detection of the HIV-1 antigen by using monoclonal antibodies to protein p24]. Voprosy virusologii 1992. PMID: 1290220


  59. Filatov FP, Tugizov ShM, Savchenkova IP, Kumarev VP, Belikov AS, Kadoshnikov IuP, Kush AA, Uryvaev LV. [Recombinant surface proteins of the hepatitis B virus, exhibiting the immunodominant membrane protein of the HIV-1 virus]. Doklady Akademii nauk 1992. PMID: 1486344


  60. El'bert LB, Vorovich MF, Terletskaia EN, Lisitsina EA, Atanadze SN, Sidorovich IG, Khozinskii VV, Tugizov ShM, Kushch AA, Khapchaev IuKh. [The protective activity of preparations made from the tick-borne encephalitis virus grown using different cell cultures]. Zhurnal mikrobiologii, epidemiologii, i immunobiologii 1992. PMID: 1414102


  61. Shpilevaia MV, Tugizov ShM, Kushch AA, Tsilinskii IaIa, Makarova NE. [The biological and immunochemical properties of monoclonal antibodies to the Venezuelan equine encephalomyelitis virus]. Voprosy virusologii 1991. PMID: 1723821


  62. Kushch AA, Tugizov ShM, Simonov VI, Timofeev IV, Perminova NP, Shumai EP. [The properties of monoclonal antibodies to HIV gag gene proteins]. Voprosy virusologii 1991. PMID: 1796579


  63. Vorovich MF, Timofeev AV, Atanadze SN, Tugizov ShM, Sidorovich IG, Khozinskii VV, Kushch AA, El'bert LB. [The inhibition of the pH-dependent fusion of the tick-borne encephalitis virus with artificial membranes by using non-neutralizing monoclonal antibodies]. Doklady Akademii nauk SSSR 1991. PMID: 2055152


  64. Vorovitch MF, Timofeev AV, Atanadze SN, Tugizov SM, Kushch AA, Elbert LB. pH-dependent fusion of tick-borne encephalitis virus with artificial membranes. Archives of virology 1991. PMID: 2048972


  65. A. V. Timofeev, Alla A. Kushch, M. F. Vorovitch, S. M. Tugizov, L. B. Elbert, D. K. Lvov. A study of the NS3 nonstructural protein of tick-borne encephalitis virus using monoclonal antibodies against the virus. 1990. PMID:


  66. Kushch AA, Beliavskii AV, Shumai EP, Tugizov ShM, Ovcharenko AV. [Monoclonal antibodies against topoisomerase I inhibit cell entering into the phase of DNA synthesis]. Doklady Akademii nauk SSSR 1988. PMID: 2844486