Barbara Bensing, PhD

Associate Adjunct Professor

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Education
PhD, 10/1996 - Microbiology, University of Minnesota
MS, 11/1992 - Microbial Engineering, University of Minnesota
BS, 04/1984 - Cell and Molecular Biology, University of Michigan
Publications

  1. KeAndreya Morrison, Barbara Bensing, Hai Yu, Xi Chen, Paul Sullam, T. Iverson. Abstract 1982 Targeting Sialic Acids with Streptococcal Adhesin Proteins. Journal of Biological Chemistry 2024. PMID:


  2. Di Carluccio C, Cerofolini L, Moreira M, Rosu F, Padilla-Cortés L, Gheorghita GR, Xu Z, Santra A, Yu H, Yokoyama S, Gray TE, St Laurent CD, Manabe Y, Chen X, Fukase K, Macauley MS, Molinaro A, Li T, Bensing BA, Marchetti R, Gabelica V, Fragai M, Silipo A. Molecular Insights into O-Linked Sialoglycans Recognition by the Siglec-Like SLBR-N (SLBRUB10712) of Streptococcus gordonii. ACS central science 2024. PMID: 38435526


  3. Heindel D, Acosta DF, Goff M, Jan M, Wang XH, Petrova M, Chan KW, Kong XP, Chen B, Mahal L, Bensing B, Hioe C. HIV-1 interaction with an O-glycan-specific bacterial lectin enhances virus infectivity and cell-to-cell viral transfer. Research square 2023. PMID: 36824869


  4. Srivastava S, Verhagen A, Sasmal A, Wasik BR, Diaz S, Yu H, Bensing BA, Khan N, Khedri Z, Secrest P, Sullam P, Varki N, Chen X, Parrish CR, Varki A. Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome. Glycobiology 2022. PMID: 35926090


  5. Bensing BA, Stubbs HE, Agarwal R, Yamakawa I, Luong K, Solakyildirim K, Yu H, Hadadianpour A, Castro MA, Fialkowski KP, Morrison KM, Wawrzak Z, Chen X, Lebrilla CB, Baudry J, Smith JC, Sullam PM, Iverson TM. Origins of glycan selectivity in streptococcal Siglec-like adhesins suggest mechanisms of receptor adaptation. Nature communications 2022. PMID: 35585145


  6. Daniel W. Heindel, Kun-Wei Chan, Mariya I. Petrova, Xiang-Peng Kong, Sarah Lebeer, Barbara A. Bensing, Lara K. Mahal, Catarina E. Hioe. Biological Consequences of HIV-1 Interactions with Bacteria. The FASEB Journal 2022. PMID:


  7. Walker MR, Goel HL, Mukhopadhyay D, Chhoy P, Karner ER, Clark JL, Liu H, Li R, Zhu JL, Chen S, Mahal LK, Bensing BA, Mercurio AM. O-linked α2,3 sialylation defines stem cell populations in breast cancer. TO REMOVE 2022. PMID: 34995107


  8. Solakyildirim K, Li Y, Bayer AS, Sullam PM, Xiong YQ, Lebrilla CB, Bensing BA. Proteoglycan 4 (lubricin) is a highly sialylated glycoprotein associated with cardiac valve damage in animal models of infective endocarditis. Glycobiology 2021. PMID: 34459483


  9. Di Carluccio C, Forgione RE, Bosso A, Yokoyama S, Manabe Y, Pizzo E, Molinaro A, Fukase K, Fragai M, Bensing BA, Marchetti R, Silipo A. Molecular recognition of sialoglycans by streptococcal Siglec-like adhesins: toward the shape of specific inhibitors. TO REMOVE 2021. PMID: 34977577


  10. Nason R, Büll C, Konstantinidi A, Sun L, Ye Z, Halim A, Du W, Sørensen DM, Durbesson F, Furukawa S, Mandel U, Joshi HJ, Dworkin LA, Hansen L, David L, Iverson TM, Bensing BA, Sullam PM, Varki A, Vries E, de Haan CAM, Vincentelli R, Henrissat B, Vakhrushev SY, Clausen H, Narimatsu Y. Display of the human mucinome with defined O-glycans by gene engineered cells. Nature communications 2021. PMID: 34210959


  11. Bhagirath D, Liston M, Akoto T, Lui B, Bensing BA, Sharma A, Saini S. Novel, non-invasive markers for detecting therapy induced neuroendocrine differentiation in castration-resistant prostate cancer patients. TO REMOVE 2021. PMID: 33859239


  12. Seepersaud R, Anderson AC, Bensing BA, Choudhury BP, Clarke AJ, Sullam PM. O-acetylation controls the glycosylation of bacterial serine-rich repeat glycoproteins. The Journal of biological chemistry 2021. PMID: 33384382


  13. Agarwal R, Bensing BA, Mi D, Vinson PN, Baudry J, Iverson TM, Smith JC. Structure based virtual screening identifies small molecule effectors for the sialoglycan binding protein Hsa. TO REMOVE 2020. PMID: 32910185


  14. Stubbs HE, Bensing BA, Yamakawa I, Sharma P, Yu H, Chen X, Sullam PM, Iverson TM. Tandem sialoglycan-binding modules in a Streptococcus sanguinis serine-rich repeat adhesin create target dependent avidity effects. The Journal of biological chemistry 2020. PMID: 32820052


  15. Miriam Braunstein, Barbara A. Bensing, Paul M. Sullam. The Two Distinct Types of SecA2-Dependent Export Systems. TO REMOVE 2019. PMID:


  16. Bensing BA, Li L, Yakovenko O, Wong M, Barnard KN, Iverson TM, Lebrilla CB, Parrish CR, Thomas WE, Xiong Y, Sullam PM. Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection. PLoS pathogens 2019. PMID: 31233555


  17. Narimatsu Y, Joshi HJ, Nason R, Van Coillie J, Karlsson R, Sun L, Ye Z, Chen YH, Schjoldager KT, Steentoft C, Furukawa S, Bensing BA, Sullam PM, Thompson AJ, Paulson JC, Büll C, Adema GJ, Mandel U, Hansen L, Bennett EP, Varki A, Vakhrushev SY, Yang Z, Clausen H. An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells. Molecular cell 2019. PMID: 31227230


  18. Saurabh Srivastava, Andrea Verhagen, Brian Wasik, Hai Yu, Aniruddha Sasmal, Barbara Bensing, Naazneen Khan, Zahra Khedri, Sandra Diaz, Paul Sullam, Nissi Varki, Xi Chen, Colin Parrish, Ajit Varki. Development of Sialoglycan Recognizing Probes (SGRPs) With Defined Specificities: Towards Exploring the Dynamic Mammalian Sialoglycome. The Journal of Immunology 2019. PMID:


  19. Braunstein M, Bensing BA, Sullam PM. The Two Distinct Types of SecA2-Dependent Export Systems. Microbiology spectrum 2019. PMID: 31215505


  20. Malaker SA, Pedram K, Ferracane MJ, Bensing BA, Krishnan V, Pett C, Yu J, Woods EC, Kramer JR, Westerlind U, Dorigo O, Bertozzi CR. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. TO REMOVE 2019. PMID: 30910957


  21. Spencer C, Bensing BA, Mishra NN, Sullam PM. Membrane trafficking of the bacterial adhesin GspB and the accessory Sec transport machinery. The Journal of biological chemistry 2018. PMID: 30514759


  22. Bensing BA, Li Q, Park D, Lebrilla CB, Sullam PM. Streptococcal Siglec-like Adhesins Recognize Different Subsets of Human Plasma Glycoproteins: Implications for Infective Endocarditis. Glycobiology 2018. PMID: 29796594


  23. Yakovenko O, Nunez J, Bensing B, Yu H, Mount J, Zeng J, Hawkins J, Chen X, Sullam PM, Thomas W. SRR Adhesins Mediate Shear-Enhanced Streptococcal Binding to Platelets. Infection and immunity 2018. PMID: 29581195


  24. Chen Y, Bensing BA, Seepersaud R, Mi W, Liao M, Jeffrey PD, Shajahan A, Sonon RN, Azadi P, Sullam PM, Rapoport TA. Unraveling the sequence of cytosolic reactions in the export of GspB adhesin from Streptococcus gordonii. The Journal of biological chemistry 2018. PMID: 29462788


  25. Seepersaud R, Sychantha D, Bensing BA, Clarke AJ, Sullam PM. O-acetylation of the serine-rich repeat glycoprotein GspB is coordinated with accessory Sec transport. Volume 13 of Issue 8. PLoS pathogens 2017. PMID: 28827841


  26. Loukachevitch LV, Bensing BA, Yu H, Zeng J, Chen X, Sullam PM, Iverson TM. Structures of the Streptococcus sanguinis SrpA Binding Region with Human Sialoglycans Suggest Features of the Physiological Ligand. Biochemistry 2016. PMID: 27685666


  27. Bensing BA, Khedri Z, Deng L, Yu H, Prakobphol A, Fisher SJ, Chen X, Iverson TM, Varki A, Sullam PM. Novel aspects of sialoglycan recognition by the Siglec-like domains of streptococcal SRR glycoproteins. Volume 26 of Issue 11. Glycobiology 2016. PMID: 27037304


  28. Chen Y, Seepersaud R, Bensing BA, Sullam PM, Rapoport TA. Mechanism of a cytosolic O-glycosyltransferase essential for the synthesis of a bacterial adhesion protein. Volume 113 of Issue 9. Proceedings of the National Academy of Sciences of the United States of America 2016. PMID: 26884191


  29. Bensing BA, Loukachevitch LV, McCulloch KM, Yu H, Vann KR, Wawrzak Z, Anderson S, Chen X, Sullam PM, Iverson TM. Structural Basis for Sialoglycan Binding by the Streptococcus sanguinis SrpA Adhesin. Volume 291 of Issue 14. The Journal of biological chemistry 2016. PMID: 26833566


  30. Deng L, Bensing BA, Thamadilok S, Yu H, Lau K, Chen X, Ruhl S, Sullam PM, Varki A. Oral streptococci utilize a Siglec-like domain of serine-rich repeat adhesins to preferentially target platelet sialoglycans in human blood. Volume 10 of Issue 12. PLoS pathogens 2014. PMID: 25474103


  31. Bensing BA, Seepersaud R, Yen YT, Sullam PM. Selective transport by SecA2: an expanding family of customized motor proteins. Volume 1843 of Issue 8. Biochimica et biophysica acta 2013. PMID: 24184206


  32. Yen YT, Cameron TA, Bensing BA, Seepersaud R, Zambryski PC, Sullam PM. Differential localization of the streptococcal accessory sec components and implications for substrate export. Volume 195 of Issue 4. Journal of bacteriology 2012. PMID: 23204472


  33. Seepersaud R, Bensing BA, Yen YT, Sullam PM. The accessory Sec protein Asp2 modulates GlcNAc deposition onto the serine-rich repeat glycoprotein GspB. Volume 194 of Issue 20. Journal of bacteriology 2012. PMID: 22885294


  34. Bensing BA, Yen YT, Seepersaud R, Sullam PM. A Specific interaction between SecA2 and a region of the preprotein adjacent to the signal peptide occurs during transport via the accessory Sec system. Volume 287 of Issue 29. The Journal of biological chemistry 2012. PMID: 22654116


  35. Pyburn TM, Bensing BA, Xiong YQ, Melancon BJ, Tomasiak TM, Ward NJ, Yankovskaya V, Oliver KM, Cecchini G, Sulikowski GA, Tyska MJ, Sullam PM, Iverson TM. A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors. Volume 7 of Issue 7. PLoS pathogens 2011. PMID: 21765814


  36. Yen YT, Seepersaud R, Bensing BA, Sullam PM. Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System. Volume 193 of Issue 13. Journal of bacteriology 2011. PMID: 21531800


  37. Pyburn TM, Yankovskaya V, Bensing BA, Cecchini G, Sullam PM, Iverson TM. Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding region of the Streptococcus gordonii adhesin GspB. Volume 66 of Issue Pt 11. Acta crystallographica. Section F, Structural biology and crystallization communications 2010. PMID: 21045307


  38. Seepersaud R, Bensing BA, Yen YT, Sullam PM. Asp3 mediates multiple protein-protein interactions within the accessory Sec system of Streptococcus gordonii. Volume 78 of Issue 2. Molecular microbiology 2010. PMID: 20807195


  39. Bensing BA, Sullam PM. Transport of preproteins by the accessory Sec system requires a specific domain adjacent to the signal peptide. Volume 192 of Issue 16. Journal of bacteriology 2010. PMID: 20562303


  40. Bensing BA, Sullam PM. Characterization of Streptococcus gordonii SecA2 as a paralogue of SecA. Volume 191 of Issue 11. Journal of bacteriology 2009. PMID: 19363114


  41. Xiong YQ, Bensing BA, Bayer AS, Chambers HF, Sullam PM. Role of the serine-rich surface glycoprotein GspB of Streptococcus gordonii in the pathogenesis of infective endocarditis. Volume 45 of Issue 4. Microbial pathogenesis 2008. PMID: 18656529


  42. Bensing BA, Siboo IR, Sullam PM. Glycine residues in the hydrophobic core of the GspB signal sequence route export toward the accessory Sec pathway. Volume 189 of Issue 10. Journal of bacteriology 2007. PMID: 17369296


  43. Takamatsu D, Bensing BA, Prakobphol A, Fisher SJ, Sullam PM. Binding of the streptococcal surface glycoproteins GspB and Hsa to human salivary proteins. Volume 74 of Issue 3. Infection and immunity 2006. PMID: 16495569


  44. Bensing BA, Takamatsu D, Sullam PM. Determinants of the streptococcal surface glycoprotein GspB that facilitate export by the accessory Sec system. Volume 58 of Issue 5. Molecular microbiology 2005. PMID: 16313630


  45. Takamatsu D, Bensing BA, Cheng H, Jarvis GA, Siboo IR, López JA, Griffiss JM, Sullam PM. Binding of the Streptococcus gordonii surface glycoproteins GspB and Hsa to specific carbohydrate structures on platelet membrane glycoprotein Ibalpha. Volume 58 of Issue 2. Molecular microbiology 2005. PMID: 16194227


  46. Takamatsu D, Bensing BA, Sullam PM. Two additional components of the accessory sec system mediating export of the Streptococcus gordonii platelet-binding protein GspB. Volume 187 of Issue 11. Journal of bacteriology 2005. PMID: 15901716


  47. Takamatsu D, Bensing BA, Sullam PM. Four proteins encoded in the gspB-secY2A2 operon of Streptococcus gordonii mediate the intracellular glycosylation of the platelet-binding protein GspB. Volume 186 of Issue 21. Journal of bacteriology 2004. PMID: 15489421


  48. Bensing BA, López JA, Sullam PM. The Streptococcus gordonii surface proteins GspB and Hsa mediate binding to sialylated carbohydrate epitopes on the platelet membrane glycoprotein Ibalpha. Volume 72 of Issue 11. Infection and immunity 2004. PMID: 15501784


  49. Takamatsu D, Bensing BA, Sullam PM. Genes in the accessory sec locus of Streptococcus gordonii have three functionally distinct effects on the expression of the platelet-binding protein GspB. Volume 52 of Issue 1. Molecular microbiology 2004. PMID: 15049820


  50. Bensing BA, Gibson BW, Sullam PM. The Streptococcus gordonii platelet binding protein GspB undergoes glycosylation independently of export. Volume 186 of Issue 3. Journal of bacteriology 2004. PMID: 14729688


  51. Siboo IR, Bensing BA, Sullam PM. Genomic organization and molecular characterization of SM1, a temperate bacteriophage of Streptococcus mitis. Volume 185 of Issue 23. Journal of bacteriology 2003. PMID: 14617660


  52. Bensing BA, Sullam PM. An accessory sec locus of Streptococcus gordonii is required for export of the surface protein GspB and for normal levels of binding to human platelets. Volume 44 of Issue 4. Molecular microbiology 2002. PMID: 12010500


  53. Bensing BA, Siboo IR, Sullam PM. Proteins PblA and PblB of Streptococcus mitis, which promote binding to human platelets, are encoded within a lysogenic bacteriophage. Infection and immunity 2001. PMID: 11553559


  54. Bensing BA, Rubens CE, Sullam PM. Genetic loci of Streptococcus mitis that mediate binding to human platelets. Infection and immunity 2001. PMID: 11179301


  55. Bensing BA, Manias DA, Dunny GM. Pheromone cCF10 and plasmid pCF10-encoded regulatory molecules act post-transcriptionally to activate expression of downstream conjugation functions. TO REMOVE 1997. PMID: 9159516


  56. Bensing BA, Dunny GM. Pheromone-inducible expression of an aggregation protein in Enterococcus faecalis requires interaction of a plasmid-encoded RNA with components of the ribosome. TO REMOVE 1997. PMID: 9159517


  57. Bensing BA, Meyer BJ, Dunny GM. Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis. TO REMOVE 1996. PMID: 8755555


  58. Chung JW, Bensing BA, Dunny GM. Genetic analysis of a region of the Enterococcus faecalis plasmid pCF10 involved in positive regulation of conjugative transfer functions. TO REMOVE 1995. PMID: 7721703


  59. Leonard BA, Bensing BA, Hedberg PJ, Ruhfel RE, Chung JW, Dunny GM. Pheromone-inducible gene regulation and signalling for the control of aggregation substance expression in the conjugative plasmid pCF10. TO REMOVE 1995. PMID: 8586188


  60. Bensing BA, Dunny GM. Cloning and molecular analysis of genes affecting expression of binding substance, the recipient-encoded receptor(s) mediating mating aggregate formation in Enterococcus faecalis. TO REMOVE 1993. PMID: 8226689