Volume 29 | Haematology and blood transfusion
Authors: Greene WC, Depper JM, Crabtree GR, Rudikoff S, Pumphrey J, Robb RJ, Krönke M, Svetlik P, Peffer NJ, Waldmann TA
The role of protein kinase C, and effects of 5-azacytidine on regulation on interleukin-2 receptor expression
Authors: Depper JM, Leonard WJ, Kronke M, Waldmann TA, Greene WC
Cloning of cDNAs for the human interleukin-2 receptor and determination of its complete protein sequence
Authors: Leonard WJ, Depper JM, Crabtree GR, Rudikoff S, Pumphrey J, Robb RJ, Kronke M, Svetlik P, Peffer NJ, Waldmann TA, Greene WC
Cyclosporin A inhibits expression of select T-cell activation genes
Authors: Kronke M, Depper JM, Leonard WJ, Wong-Staal F, Gallo RC, Waldmann TA, Greene WC
Interleukin-2 receptors on lymphocytes
Authors: Waldmann TA, Leonard WJ, Depper JM, Kronke M, Goldman CK, Sharrow S, Greene WC
Molecular analysis of the human interleukin-2 receptor
Authors: Greene WC, Depper JM, Crabtree GR, Rudikoff S, Pumphrey J, Robb RJ, Kronke M, Svetlik P, Peffer NJ, Waldmann TA, Leonard WJ
Molecular cloning and expression of cDNAs encoding the human interleukin-2 receptor
Authors: Leonard WJ, Depper JM, Crabtee GM, Rudikoff S, Pumphrey J, Robb RJ, Kronke M, Svetlik P, Peffer NJ, Waldmann TA, Greene WC
Interleukin-2 receptors
Authors: Waldmann TA, Leonard WJ, Depper JM, Kronke M, Goldman CK, Bongiovani KF, Greene WC.
Structure, function, and expression of the receptor for interleukin-2 on normal and malignant lymphocytes
Authors: Waldmann TA, Leonard WJ, Depper JM, Kronke M, Kozak R, Greene WC
Volume 134 of Issue 1 | Journal of immunology (Baltimore, Md. : 1950)
Authors: Reed JC, Robb RJ, Greene WC, Nowell PC
Wheat germ agglutinin (WGA) inhibits proliferation of human peripheral blood mononuclear cells (PBMC) induced by mitogens and antigens. We investigated the mechanism by which WGA inhibits PHA-induced human lymphocyte proliferation with regard to the interleukin pathway. Our data revealed that although PBMC-proliferation was markedly suppressed by WGA, levels of IL 2 activity in WGA-inhibited cultures were not reduced, but instead were increased, suggesting failure to utilize IL 2. Furthermore, the addition of exogenous IL 2 failed to overcome the suppression. Consistent with these observations, culturing PBMC with PHA plus WGA markedly decreased the number of high-affinity IL 2 receptor per cell, as determined by binding of purified [3H]IL 2, relative to cultures containing PHA alone. WGA immobilized on support beads bound detergent-solubilized IL 2 receptors from PHA-activated T cells, but did not bind human IL 2. However, WGA did not competitively block the binding of [3H]IL 2 to PHA-induced lymphoblasts. These results suggest that WGA inhibits lymphocyte proliferation by binding to and decreasing the number of high-affinity IL 2 receptors displayed on T cells, without impairing IL 2 production.
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