The Science of the total environment
Authors: Berger K, Coker E, Rauch S, Eskenazi B, Balmes J, Kogut K, Holland N, Calafat AM, Harley K
Journal of clinical microbiology
Authors: Mehaffy C, Kruh-Garcia NA, Graham B, Jarlsberg LG, Willyerd CE, Borisov A, Sterling TR, Nahid P, Dobos KM
Identification of biomarkers for latent infection and risk of progression to tuberculosis (TB) disease are needed to better identify individuals to target for preventive therapy, predict disease risk, and potentially predict preventive therapy efficacy. Our group developed Multiple Reaction Monitoring Mass Spectrometry (MRM-MS) assays that detected ) peptides in serum extracellular vesicles from TB patients. We subsequently optimized this MRM-MS assay to selectively identify 40 peptides from 19 proteins that most commonly co-purify with serum vesicles of patients with TB. Here, we used this technology to evaluate if peptides can also be detected in individuals with latent TB infection (LTBI). Serum extracellular vesicles from 74 individuals presumed to have latent infection (LTBI) based on close contact with a household member with TB or a recent tuberculin skin test (TST) conversion were included in this study. Twenty-nine samples from individuals with no evidence of TB infection by TST and no known exposure to TB were used as controls to establish a threshold to account for non-specific/background signal. We identified at least one of the 40 peptides in 70 (95%) individuals with LTBI. A single peptide from the Glutamine synthetase (GlnA1) enzyme was identified in 61/74 (82%) individuals with LTBI, suggesting peptides from proteins involved in nitrogen metabolism as candidates for pathogen specific biomarkers for detection of LTBI. The detection of peptides in serum extracellular vesicles from persons with LTBI represents a potential advance in the diagnosis of LTBI.
View on PubMed
BMC musculoskeletal disorders
Authors: Singh S, Kidane J, Wentworth KL, Motamedi D, Morshed S, Schober AE, Hsiao EC
Cancer research
Authors: Marker KM, Zavala VA, Vidaurre T, Lott PC, Vásquez JN, Casavilca-Zambrano S, Calderón M, Abugattas JE, Gómez HL, Fuentes HA, Picoaga RL, Cotrina JM, Neciosup SP, Castañeda CA, Morante Z, Valencia F, Torres J, Echeverry M, Bohórquez ME, Polanco-Echeverry G, Estrada-Florez AP, Serrano-Gómez SJ, Carmona-Valencia JA, Alvarado-Cabrero I, Sanabria-Salas MC, Velez A, Donado J, Song S, Cherry D, Tamayo LI, Huntsman S, Hu D, Ruiz-Cordero R, Balassanian R, Ziv E, Zabaleta J, Carvajal-Carmona L, Fejerman L
American journal of respiratory and critical care medicine
Authors: Crotty Alexander LE, Ware LB, Calfee CS, Callahan SJ, Eissenberg T, Farver C, Goniewicz ML, Jaspers I, Kheradmand F, King TE, Meyer NJ, Mikheev V, Shields PG, Shihadeh A, Strongin R, Tarran R
The American journal of cardiology
Authors: Kolte D, Kennedy KF, Passeri JJ, Inglessis I, Elmariah S
Authors: Kigozi SP, Giorgi E, Mpimbaza A, Kigozi RN, Bousema T, Arinaitwe E, Nankabirwa JI, Sebuguzi CM, Kamya MR, Staedke SG, Dorsey G, Pullan RL
The Journal of molecular diagnostics : JMD
Authors: Adamson PC, Pandori MW, Doernberg SB, Komarow L, Sund Z, Tran TTT, Jensen D, Tsalik EL, Deal CD, Chambers HF, Fowler VG, Evans SR, Patel R, Klausner JD
Cornea
Authors: Gonzales JA, Shiboski SC, Bunya VY, Akpek EK, Rose-Nussbaumer J, Seitzman GD, Criswell LA, Shiboski CH, Lietman TM
Journal of general internal medicine
Authors: Weaver FM, Hickok A, Prasad B, Tarlov E, Zhang Q, Taylor A, Bartle B, Gordon H, Young R, Sarmiento K, Hynes DM
