|
p:
415-476-4116
f: 415-476-1291
e: Hyewon.Phee@ucsf.edu
University
of California, San Francisco
533 Parnassus Avenue, Room S-1032
San Francisco, CA. 94143-0795
Education
1993: Bachelor, Seoul National University, Seoul, Korea
1996: M.S., Dept. of Chemistry, Seoul National University, Seoul, Korea 1996- 2001: Ph.D., Dept. of Biochemistry, Ohio State University
Honors
7/1/2006-6/30/2009: Career Development Program Special Fellow Award --The Leukemia and Lymphoma Society, USA
1996-1999: National Study Abroad Scholarship, Korea
Research Interests
Understanding the basic mechanism of how immune cells are activated and regulated is pivotal to the development of new therapies for immunological diseases such as autoimmunity, leukemia and lymphoma. Immune cells are activated following antigen recognition via their receptors. My research focuses on one of the critical signaling pathways that is activated upon T cell receptor engagement. More specifically, I am interested in the pathway that activates PAK (p21-activated kinases) and their binding partners, GIT (G protein-coupled receptor kinase-interacting target) and PIX (PAK interacting exchange factor). Although this pathway is thought to play an important role in T cell activation and cytoskeletal dynamics and eventually generate productive immune responses, the exact function and activation mechanism is not clear. Thus, I will define the role and activation mechanism of this pathway in the immune system by combining biochemical and genetic approaches. Since alteration of this pathway has been reported in various types of cancer, including leukemia and lymphoma, manipulation of this pathway may promote cancer cell death and tumor regression. Therefore, characterization of this signaling pathway may lead to the development of compounds that may be effective in cancer treatment. In addition, my research will clarify the importance of the PAK, GIT and PIX in normal T cell activation and cytoskeletal dynamics and may provide insights into mechanisms relevant to T cell autoimmunity that could lead to novel therapeutics.
Publications
- Schmalzigaug R, Phee H , Davidson CE, Weiss A, Premont RT Journal of Histochemistry and Cytochemistry , 2007 Jun 12, Expression of the ARF GAP genes GIT1 and GIT2 in different mouse tissues.
- Nirula A, Ho M, Phee H, Roose J, Weiss A, J Exp Med. 2006 Jul 10;203(7):1733-44. Epub 2006 Jun 19, Phosphoinositide-dependent kinase 1 targets protein kinase A in a pathway that regulates interleukin 4.
- Yamashita Y, Phee H, Tudor KS, Rossi MI, Parnes JR, Coggeshall KM, Kincade PW, Hybridoma(Larchmt). 2006 Jun;25(3):107-14. A unique CD72 epitope suggests a potential interaction with Fc gamma RII/CD32 on B lineage lymphocytes.
- Phee H , Abraham RT, Weiss A, Nature Immunology, 2005, Jun;6(6):608-17. Epub 2005 May 1. Dynamic recruitment of PAK1 to the immunological synapse is mediated by PIX independently of SLP-76 and Vav1.
- Vedham V, Phee H, Coggeshall KM, Mol Cell Biol 2005, May;25(10):4211-20. Vav activation and function as a rac Guanine nucleotide exchange factor in macrophage colony-stimulating factor-induced macrophage chemotaxis.
- Fanzo JC, Lynch MP, Phee H, Hyer M, Cremesti A, Grassme H, Norris JS, Coggeshall KM, Rueda BR, Pernis AB , Kolesnick R, Gulbins E, Cancer Biol Ther 2003 Jul-Aug;2(4):392-5. CD95 rapidly clusters in cells of diverse origins.
- Coggeshall KM, Nakamura K, Phee H. Mol Immunol 2002 Dec;39(9):521-9. How do inhibitory phosphatases work?
- Phee H , Rodgers W, Coggeshall KM. Mol Cell Biol 2001 Dec;21(24):8615-25. Visualization of negative signaling in B cells by quantitative confocal microscopy.
- Cooney D, Phee H, Jacob A, Coggeshall KM. J Immunol 2001 Jul 15;167(2):844-54. Signal transduction by human-restricted FcgRIIa involves three distinct cytoplasmic kinase families leading to phagocytosis.
- Phee H , Coggeshall KM. Inhibitory signal transduction by FcgRII in: Recent Research Developments in Molecular & Cellular Biology, 2 (2001):141-161 ISBN:81-7736-047-7, ed. Pandalai S.G., Research signpost, Trivandrum, India
- Phee H , Jacob A, Coggeshall KM. J Biol Chem 2000 ;275(25):19090-7. Enzymatic activity of the Src homology 2 domain-containing inositol phosphatase is regulated by a plasma membrane location.
- Tridandapani S, Phee H, Shivakumar L, Kelley TW, Coggeshall KM. Mol Immunol1998 Dec; 35(17):1135-46. Role of SHIP in FcgammaRIIb-mediated inhibition of Ras activation in B cells.
|
