Common Laboratory Protocols

Antibodies

  • Antibody binding to Protein A/G
  • FACS for BMDM
  • Isotypes of commonly used antibodies

    Cell purifications and assays

  • Human PMN Purification
  • Murine PMN from peripheral blood
  • Murine PMN from bone marrow
  • MEF preparation
  • Retroviral transduction of bone marrow (from Mary Dinauer)
  • Retroviral infection of BMDM
  • T cell- APC conjugate analysis
  • Phagocytosis of apoptotic Jurkats by bone marrow-derived macrophages

    Biochemistry

  • Sigma buffer chart
  • Recombinant protein refolding
  • Purification and labeling of GPI-anchored proteins (from Mike Dustin)
  • Preparation of GPI-anchored protein-containing liposomes (from Mike Dustin)

    Mycobacteria

  • Selecting transposon-containing M. marinum
  • Transposon insertion site sequence by inverse PCR